Secretory protein trafficking and organelle dynamics in living cells

J. Lippincott-Schwartz*, T. H. Roberts, K. Hirschberg

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


Green fluorescent protein chimerae acting as reporters for protein localization and trafficking within the secretory membrane system of living cells have been used in a wide variety of applications, including time-lapse imaging, double-labeling, energy transfer, quantitation, and photobleaching experiments. Results from this work are clarifying the steps involved in the formation, translocation, and fusion of transport intermediates; the organization and biogenesis of organelles; and the mechanisms of protein retention, sorting, and recycling in the secretory pathway. In so doing, they are broadening our thinking about the temporal and spatial relationships among secretory organelles and the membrane trafficking pathways that operate between them.

Original languageEnglish
Pages (from-to)557-589
Number of pages33
JournalAnnual Review of Cell and Developmental Biology
StatePublished - 2000
Externally publishedYes


  • Endoplasmic reticulum
  • Golgi complex
  • Green fluorescent protein
  • Protein trafficking


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