Scanning electron microscopic delineation of bacterial surface topology using cationized ferritin

Raphael Lamed, Jenny Naimark, Ely Morgenstern, Edward A. Bayer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A novel method is presented for the labelling of negatively charged bacterial cell surface structures which allows their ultrastructural observation at the scanning electron microscopic level of resolution. The method is based on treatment of bacterial cells with cationized ferritin prior to examination. The method is simple to perform, rapid, reproducible and inexpensive. The approach provides a new topographical view of the fine structure of the bacterial cell surface.

Original languageEnglish
Pages (from-to)233-240
Number of pages8
JournalJournal of Microbiological Methods
Volume7
Issue number4-5
DOIs
StatePublished - 28 Dec 1987

Keywords

  • Bacterial surface topology
  • Cationized ferritin
  • Exocellular structure
  • Scanning electron microscopy

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