TY - JOUR
T1 - Saturable [D-Ala2, D-Leu5]-enkephalin transport into cholinergic synaptic vesicles
AU - Day, Nicola C.
AU - Wien, Daniela
AU - Michaelson, Daniel M.
N1 - Funding Information:
We thank Dr Y. Sarnef or stimulatinga nd fruitful discussionsa nd Dr Riedel of the Squibb Institute for Medical Researchf or a gift of Cap-‘opril. This work was supportedi n part by grants from the US-Israel Binational ScienceF oundation (grant no.2410)a nd the Fund for Basic Research administeredb y the Israel Academy of Sciences and Humanities.
PY - 1985/4/8
Y1 - 1985/4/8
N2 - Incubation of cholinergic synaptic vesicles purified from the electric organ of Torpedo ocellata with radiolabeled [D-Ala2, D-Leu5]-enkephalin results in its accumulation in the vesicles. Kinetic analysis of the initial rates of uptake reveals temperature-dependent saturation kinetics which are best fitted by a single transport system (KT = 12 ± 2 μM and Vmax = 0.85 ± 0.24 pmol mg protein per min). The specific rates and extents of [3H]-[D-Ala2, D-Leu5]-enkephalin uptake, like those of [3H]acetylcholine uptake, are highest in the purified synaptic vesicles fraction. These findings suggest that Torpedo cholinergic synaptic vesicles contain an opioid peptide transporter. The physiological significance of this transporter is discussed in view of the recent observation that Torpedo nerve terminals contain an endogenous enkephalin-like peptide and presynaptic opiate receptors. Enkephalin uptake Synaptic vesicle Acetylcholine Torpedo.
AB - Incubation of cholinergic synaptic vesicles purified from the electric organ of Torpedo ocellata with radiolabeled [D-Ala2, D-Leu5]-enkephalin results in its accumulation in the vesicles. Kinetic analysis of the initial rates of uptake reveals temperature-dependent saturation kinetics which are best fitted by a single transport system (KT = 12 ± 2 μM and Vmax = 0.85 ± 0.24 pmol mg protein per min). The specific rates and extents of [3H]-[D-Ala2, D-Leu5]-enkephalin uptake, like those of [3H]acetylcholine uptake, are highest in the purified synaptic vesicles fraction. These findings suggest that Torpedo cholinergic synaptic vesicles contain an opioid peptide transporter. The physiological significance of this transporter is discussed in view of the recent observation that Torpedo nerve terminals contain an endogenous enkephalin-like peptide and presynaptic opiate receptors. Enkephalin uptake Synaptic vesicle Acetylcholine Torpedo.
UR - http://www.scopus.com/inward/record.url?scp=0021894986&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(85)80946-7
DO - 10.1016/0014-5793(85)80946-7
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AN - SCOPUS:0021894986
SN - 0014-5793
VL - 183
SP - 25
EP - 28
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -