Safely sorting and isolating rare sperm using dielectrophoresis by trapping the tail and simultaneously distancing the head from strong electric fields

Sholom Shuchat*, Ofer Fainaru, Shahar Kol, Gilad Yossifon

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Only a single sperm is required to fertilize an oocyte. Yet, it is often missed due the inefficiency of a manual search. Dielectrophoresis (DEP)is a proven method for sorting cells, even at minute concentrations, based on their electrical properties. We previously proposed safe method to sort sperm using DEP by distancing the head from the electrodes. A working prototype is presented and the efficiency of the system is evaluated in this research. Media exchange is also done on chip to minimize the time the sperm spends in the low conductivity buffer.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages737-738
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Externally publishedYes
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 4 Oct 20209 Oct 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period4/10/209/10/20

Keywords

  • Azoospermia
  • Dielectrophoresis (DEP)
  • Lab-on-a-chip
  • Male fertility
  • Microfluidics
  • Rare sperm

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