TY - JOUR
T1 - Role of transmethylation in the elicitation of an oxidative burst in macrophages
AU - Pick, Edgar
AU - Mizel, Diane
N1 - Funding Information:
’ This work was performed in the Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, where E.P. was a visiting scientist. 2 To whom requests for reprints should be sent at Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv 69978, Israel.
PY - 1982/9/15
Y1 - 1982/9/15
N2 - Elicited guinea pig peritoneal macrophages (MPs) respond by an oxidative burst (OB) to a variety of membrane stimulants. Evidence has recently accumulated, indicating that phospholipase A2 activation resulting in unsaturated fatty acid liberation is a prerequisite for the induction of an OB by some stimulants. We examined the effect of inhibiting adenosylmethionine-dependent phospholipid methylation on the capacity of MPs to produce superoxide (O2-) in response to membrane stimulation. We found that preincubation of MPs with the transmethylation inhibitor, 3-deazaadenosine (DZAdo), totally eliminated the induction of an OB by concanavalin A, wheat germ agglutinin, and N-formyl-l-methionyl-l-leucyl-l-phenylalanine and partially blocked O2- production in response to NaF, phospholipase C, digitonin, the ionophore A23187, and phorbol myristate acetate (PMA). The PMA-elicited OB was the most resistant to inhibition by DZAdo. Homocysteine thiolactone enhanced the blocking effect of DZAdo. These findings suggest that stimulated O2- production by guinea pig peritoneal MPs requires enzymatic methylation of an unknown substrate, most likely a membrane phospholipid.
AB - Elicited guinea pig peritoneal macrophages (MPs) respond by an oxidative burst (OB) to a variety of membrane stimulants. Evidence has recently accumulated, indicating that phospholipase A2 activation resulting in unsaturated fatty acid liberation is a prerequisite for the induction of an OB by some stimulants. We examined the effect of inhibiting adenosylmethionine-dependent phospholipid methylation on the capacity of MPs to produce superoxide (O2-) in response to membrane stimulation. We found that preincubation of MPs with the transmethylation inhibitor, 3-deazaadenosine (DZAdo), totally eliminated the induction of an OB by concanavalin A, wheat germ agglutinin, and N-formyl-l-methionyl-l-leucyl-l-phenylalanine and partially blocked O2- production in response to NaF, phospholipase C, digitonin, the ionophore A23187, and phorbol myristate acetate (PMA). The PMA-elicited OB was the most resistant to inhibition by DZAdo. Homocysteine thiolactone enhanced the blocking effect of DZAdo. These findings suggest that stimulated O2- production by guinea pig peritoneal MPs requires enzymatic methylation of an unknown substrate, most likely a membrane phospholipid.
UR - http://www.scopus.com/inward/record.url?scp=0020434117&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(82)90475-0
DO - 10.1016/0008-8749(82)90475-0
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AN - SCOPUS:0020434117
VL - 72
SP - 277
EP - 285
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 2
ER -