@article{0355ce0fb8304db891b53e0b6654b9f2,
title = "Role of the Rho GTPase Rac in the activation of the phagocyte NADPH oxidase: Outsourcing a key task",
abstract = "The superoxide-generating NADPH oxidase of phagocytes consists of the membrane-associated cytochrome b558 (a heterodimer of Nox2 and p22phox) and 4 cytosolic components: p47phox, p67phox, p40phox, and the small GTPase, Rac, in complex with RhoGDI. Superoxide is produced by the NADPH-driven reduction of molecular oxygen, via a redox gradient located in Nox2. Electron flow in Nox2 is initiated by interaction with cytosolic components, which translocate to the membrane, p67phox playing the central role.the participation of Rac is expressed in the following sequence: (1) Translocation of the RacGDP-RhoGDI complex to the membrane; (2) Dissociation of RacGDP from RhoGDI; (3) GDP to GTP exchange on Rac, mediated by a guanine nucleotide exchange factor; (4) Binding of RacGTP to p67phox; (5) Induction of a conformational change in p67phox, promoting interaction with Nox2.the particular involvement of Rac in NADPH oxidase assembly serves as a paradigm for signaling by Rho GTPases, in general.",
keywords = "Cell-free system, GEF, NADPH oxidase, Rac1, Rac2, Rho GTPases, RhoGDI, Superoxide",
author = "Edgar Pick",
note = "Funding Information: Our own research described in this report was supported by the Julius Friedrich Cohnheim-Minerva Center for Phagocyte Research, the Ela Kodesz Institute of Host Defense against Infectious Diseases, Israel Science Foundation Grants 428/01, 19/05, 49/09, and 300/13, the Roberts-Guthman Chair in Immunopharmacology, the Walter J. Levy Benevolent Trust, the Roberts Fund, the Milken-Lowell Fund, the Wallis Foundation, the Rubanenko Fund, and the Joseph and Shulamit Salomon Fund.",
year = "2014",
doi = "10.4161/sgtp.27952",
language = "אנגלית",
volume = "5",
journal = "Small GTPases",
issn = "2154-1248",
publisher = "Landes Bioscience",
number = "MAR",
}