Role of iron in transferrin-dependent lymphocyte mitogenesis in serum-free medium

Abraham Novogrodsky, Amiram Ravid, Tova Glaser, Albert L. Rubin, Kurt H. Stenzel

Research output: Contribution to journalArticlepeer-review

Abstract

Transferrin can partially replace serum requirement for the mitogenic activity of both PHA and the oxidizing mitogen, neuraminidase and galactose oxidase, whereas several other proteins are inactive. Catalase enhances the potentiating effect of transferrin, indicating that transferrin stimulates hydrogen peroxide production by the cell preparations. Iron is required for transferrin to replace serum, since prior addition of desferrioxamine to medium or cell cultures eliminates the potentiating effect of transferrin on lymphocyte mitogenesis. In addition to the possibility that iron is required for nutritional purposes, transferrin-mediated activation of oxidative metabolism may have a stimulatory effect on lymphocyte activation.

Original languageEnglish
Pages (from-to)419-422
Number of pages4
JournalExperimental Cell Research
Volume139
Issue number2
DOIs
StatePublished - Jun 1982

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