TY - JOUR
T1 - Role of iron in transferrin-dependent lymphocyte mitogenesis in serum-free medium
AU - Novogrodsky, Abraham
AU - Ravid, Amiram
AU - Glaser, Tova
AU - Rubin, Albert L.
AU - Stenzel, Kurt H.
N1 - Funding Information:
This researchw as supportedi n part by a grant from the NSF (grantP CM 7904392).
PY - 1982/6
Y1 - 1982/6
N2 - Transferrin can partially replace serum requirement for the mitogenic activity of both PHA and the oxidizing mitogen, neuraminidase and galactose oxidase, whereas several other proteins are inactive. Catalase enhances the potentiating effect of transferrin, indicating that transferrin stimulates hydrogen peroxide production by the cell preparations. Iron is required for transferrin to replace serum, since prior addition of desferrioxamine to medium or cell cultures eliminates the potentiating effect of transferrin on lymphocyte mitogenesis. In addition to the possibility that iron is required for nutritional purposes, transferrin-mediated activation of oxidative metabolism may have a stimulatory effect on lymphocyte activation.
AB - Transferrin can partially replace serum requirement for the mitogenic activity of both PHA and the oxidizing mitogen, neuraminidase and galactose oxidase, whereas several other proteins are inactive. Catalase enhances the potentiating effect of transferrin, indicating that transferrin stimulates hydrogen peroxide production by the cell preparations. Iron is required for transferrin to replace serum, since prior addition of desferrioxamine to medium or cell cultures eliminates the potentiating effect of transferrin on lymphocyte mitogenesis. In addition to the possibility that iron is required for nutritional purposes, transferrin-mediated activation of oxidative metabolism may have a stimulatory effect on lymphocyte activation.
UR - http://www.scopus.com/inward/record.url?scp=0020317741&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(82)90270-1
DO - 10.1016/0014-4827(82)90270-1
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AN - SCOPUS:0020317741
VL - 139
SP - 419
EP - 422
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -