Role of iron in transferrin-dependent lymphocyte mitogenesis in serum-free medium

Abraham Novogrodsky; Amiram Ravid; Tova Glaser; Albert L. Rubin; Kurt H. Stenzel

doi:10.1016/0014-4827(82)90270-1

Role of iron in transferrin-dependent lymphocyte mitogenesis in serum-free medium

Abraham Novogrodsky^*, Amiram Ravid, Tova Glaser, Albert L. Rubin, Kurt H. Stenzel

^*Corresponding author for this work

Clinical Departments

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Transferrin can partially replace serum requirement for the mitogenic activity of both PHA and the oxidizing mitogen, neuraminidase and galactose oxidase, whereas several other proteins are inactive. Catalase enhances the potentiating effect of transferrin, indicating that transferrin stimulates hydrogen peroxide production by the cell preparations. Iron is required for transferrin to replace serum, since prior addition of desferrioxamine to medium or cell cultures eliminates the potentiating effect of transferrin on lymphocyte mitogenesis. In addition to the possibility that iron is required for nutritional purposes, transferrin-mediated activation of oxidative metabolism may have a stimulatory effect on lymphocyte activation.

Original language	English
Pages (from-to)	419-422
Number of pages	4
Journal	Experimental Cell Research
Volume	139
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(82)90270-1
State	Published - Jun 1982

Funding

Funders	Funder number
National Science Foundation	CM 7904392

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10.1016/0014-4827(82)90270-1

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title = "Role of iron in transferrin-dependent lymphocyte mitogenesis in serum-free medium",

abstract = "Transferrin can partially replace serum requirement for the mitogenic activity of both PHA and the oxidizing mitogen, neuraminidase and galactose oxidase, whereas several other proteins are inactive. Catalase enhances the potentiating effect of transferrin, indicating that transferrin stimulates hydrogen peroxide production by the cell preparations. Iron is required for transferrin to replace serum, since prior addition of desferrioxamine to medium or cell cultures eliminates the potentiating effect of transferrin on lymphocyte mitogenesis. In addition to the possibility that iron is required for nutritional purposes, transferrin-mediated activation of oxidative metabolism may have a stimulatory effect on lymphocyte activation.",

author = "Abraham Novogrodsky and Amiram Ravid and Tova Glaser and Rubin, {Albert L.} and Stenzel, {Kurt H.}",

note = "Funding Information: This researchw as supportedi n part by a grant from the NSF (grantP CM 7904392).",

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T1 - Role of iron in transferrin-dependent lymphocyte mitogenesis in serum-free medium

AU - Novogrodsky, Abraham

AU - Ravid, Amiram

AU - Glaser, Tova

AU - Rubin, Albert L.

AU - Stenzel, Kurt H.

N1 - Funding Information: This researchw as supportedi n part by a grant from the NSF (grantP CM 7904392).

PY - 1982/6

Y1 - 1982/6

N2 - Transferrin can partially replace serum requirement for the mitogenic activity of both PHA and the oxidizing mitogen, neuraminidase and galactose oxidase, whereas several other proteins are inactive. Catalase enhances the potentiating effect of transferrin, indicating that transferrin stimulates hydrogen peroxide production by the cell preparations. Iron is required for transferrin to replace serum, since prior addition of desferrioxamine to medium or cell cultures eliminates the potentiating effect of transferrin on lymphocyte mitogenesis. In addition to the possibility that iron is required for nutritional purposes, transferrin-mediated activation of oxidative metabolism may have a stimulatory effect on lymphocyte activation.

AB - Transferrin can partially replace serum requirement for the mitogenic activity of both PHA and the oxidizing mitogen, neuraminidase and galactose oxidase, whereas several other proteins are inactive. Catalase enhances the potentiating effect of transferrin, indicating that transferrin stimulates hydrogen peroxide production by the cell preparations. Iron is required for transferrin to replace serum, since prior addition of desferrioxamine to medium or cell cultures eliminates the potentiating effect of transferrin on lymphocyte mitogenesis. In addition to the possibility that iron is required for nutritional purposes, transferrin-mediated activation of oxidative metabolism may have a stimulatory effect on lymphocyte activation.

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Role of iron in transferrin-dependent lymphocyte mitogenesis in serum-free medium

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