TY - JOUR
T1 - Role of endothelin in obstructive jaundice
AU - Sarac, A. Murat
AU - Aktan, A. Özdemir
AU - Moini, Hadi
AU - Bilsel, Serpil
AU - Scapa, Eitan
N1 - Funding Information:
Manuscript receive d September 8, 1997; accepte d July 31, 1998. From the Departme nts of Surgery and Biochemistry, Marmara University School of Medicine, Istanbul, Turkey; and Departme nts of Gastroenterology and Hepatolog, Tyel Aviv University School of Medicine,AssafHarofehHospital,Zeri® n,Israel. This work was partly supported by Marmara University Rese arch Foundation. This paper was presented in part at the 10th Annual Meeting of TheSurgicalInfectionSociety(Europe)May29± 31,1997,Istanbul and was published in abstract form in The British Journal of Su rgey r 84:887, 1997. Address for reprint requests: Dr. A. OÈ zdemir Aktan, Department of General Surgery, MrmaraaUnivrsetiHyospital,Altuni-zade, 81190 Istanbul, Turkey.
PY - 1999
Y1 - 1999
N2 - Mediators responsible for renal changes in obstructive jaundice are not specified. This study is designed to study the role of endothelin-1 (ET-1) in obstructive jaundice in rats. Animals were randomly placed into five experimental groups. Group 1 (N = 3) was the sham-operated group. Group 2 (N = 8) after common bile duct (CBD) ligation, received bosentan, which is a nonselective endothelin receptor blocker, 50 mg/kg/day for seven days. Group 3 (N = 7) received 1 μg/kg/day captopril. Group 4 (N = 7) was given both drugs orally for seven days. Group 5 (N = 6) after CBD ligation, received Arabic gum as the vehicle. Blood was drawn from the intrahepatic vena cava for the determination of ET-1, bilirubin, creatinine, protein oxidation products, hyaluronic acid, and β-N-acetyl-hexosaminase. Liver tissue samples were obtained to determine glutathione levels. ET-1, protein oxidation products, hyaluronic acid, bilirubin, and creatinine levels increased significantly in the control group when compared with sham. Bosentan effectively prevented ET-1 elevation but could not reverse creatinine or bilirubin elevation. Captopril with or without bosentan was cytoprotective but did not reverse increased creatinine levels. It is concluded that increased ET-1 in obstructive jaundice may be one of the contributing factors of renal damage.
AB - Mediators responsible for renal changes in obstructive jaundice are not specified. This study is designed to study the role of endothelin-1 (ET-1) in obstructive jaundice in rats. Animals were randomly placed into five experimental groups. Group 1 (N = 3) was the sham-operated group. Group 2 (N = 8) after common bile duct (CBD) ligation, received bosentan, which is a nonselective endothelin receptor blocker, 50 mg/kg/day for seven days. Group 3 (N = 7) received 1 μg/kg/day captopril. Group 4 (N = 7) was given both drugs orally for seven days. Group 5 (N = 6) after CBD ligation, received Arabic gum as the vehicle. Blood was drawn from the intrahepatic vena cava for the determination of ET-1, bilirubin, creatinine, protein oxidation products, hyaluronic acid, and β-N-acetyl-hexosaminase. Liver tissue samples were obtained to determine glutathione levels. ET-1, protein oxidation products, hyaluronic acid, bilirubin, and creatinine levels increased significantly in the control group when compared with sham. Bosentan effectively prevented ET-1 elevation but could not reverse creatinine or bilirubin elevation. Captopril with or without bosentan was cytoprotective but did not reverse increased creatinine levels. It is concluded that increased ET-1 in obstructive jaundice may be one of the contributing factors of renal damage.
KW - Endothelin
KW - Obstructive jaundice
KW - Renal insufficiency
UR - http://www.scopus.com/inward/record.url?scp=0032949722&partnerID=8YFLogxK
U2 - 10.1023/A:1026614803584
DO - 10.1023/A:1026614803584
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AN - SCOPUS:0032949722
VL - 44
SP - 356
EP - 363
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
SN - 0163-2116
IS - 2
ER -