The effect of adenosine triphosphate (ATP) on the first step of excision repair of ultraviolet damage in DNA has been studied using toluene treated E. coli. During postirradiation incubation, 5 to 6 times more single strand breaks are formed in DNA in the presence of exogenous ATP than in its absence. The ATP dependent as well as the ATP independent endonucleolytic activities appear to be catalyzed by the same enzyme since both activities are almost completely absent in uvrA and uvrB mutants. An ATP dependent endonucleolytic activity has been detected in nonirradiated toluene treated E. coli. It is concluded that ATP is required in vivo for either the incision step of repair or an enzymatic reaction preceding it.
|Number of pages||4|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1974|