Role of Adenosine Receptor Activation in Antioxidant Enzyme Regulation during Ischemia-Reperfusion in the Isolated Rat Heart

Edith Hochhauser, Oleg Kaminski, Hemdat Shalom, Dorit Leshem, Vladimir Shneyvays, Asher Shainberg, Bernardo A. Vidne

Research output: Contribution to journalReview articlepeer-review

Abstract

The aim of the present study was to investigate the protective role of pharmacological preconditioning on antioxidant enzymes using A1 and A3 adenosine receptor agonists in the recovery of the isolated myocardium after cardioplegic ischemia. Two different modes of preconditioning were studied: isolated rat hearts were perfused with A1 receptor agonist 2-chloro-N6-cyclopentyladenosine (CCPA) or A3 2-chloro-N6-(3-iodobenzyl) adenosine-5′-N-methyluronamide (Cl-IB-MECA) (1 nM), followed by cardioplegic ischemia and reperfusion (30 min each) (perfusion mode), or CCPA or Cl-IB-MECA (100 μg/kg) were injected intravenously 24 h before the experiment (injection mode). Hearts treated with CCPA improved in terms of mechanical function, infarct size, ATP levels, superoxide dismutase, and catalase (p < 0.005) in both modes of administration. Cl-IB-MECA was beneficial mainly in the injected group. Reduced damage to the mitochondria in the CCPA-treated hearts was observed using electron microscopy evaluation. In the Cl-IB-MECA-injected hearts, mitochondrial damage was moderate. CCPA in both modes of treatment and Cl-IB-MECA in the injected mode were beneficial in protecting the perfused isolated rat heart, subjected to normothermic cardioplegic ischemia. This protection was partially related to the higher myocardial activity of superoxide dismutase and catalase.

Original languageEnglish
Pages (from-to)335-344
Number of pages10
JournalAntioxidants and Redox Signaling
Volume6
Issue number2
DOIs
StatePublished - Apr 2004
Externally publishedYes

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