RNA sequences in the Moloney murine leukemia virus genome bound by the Gag precursor protein in the yeast three-hybrid system

Matthew J. Evans, Eran Bacharach, Stephen P. Goff*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Encapsidation of the Moloney murine leukemia virus (MMLV) genome is mediated through a specific interaction between the major viral structural protein, Gag, and an RNA packaging signal, ψ. Many studies have investigated this process in vivo, although the specific examination of the Gag-RNA interaction in this context is difficult due to the variety of other viral functions involved in virion assembly in vivo. The Saccharomyces cerevisiae three-hybrid assay was used to directly examine the interaction between MMLV Gag and ψ. In this system, MMLV RNA regions exhibiting high-affinity Gag binding were mapped. All Gag-binding regions were located 3′ to the viral splice donor sequence of the viral RNA transcript. No single short RNA sequence within ψ supported strong Gag interaction. Instead, an RNA comprised of nearly the entire ψ region was necessary to demonstrate an appreciable Gag interaction in the yeast three-hybrid system. These finding support the notion that two stem-loops (C and D) are not sufficient to form a core MMLV encapsidation signal.

Original languageEnglish
Pages (from-to)7677-7684
Number of pages8
JournalJournal of Virology
Volume78
Issue number14
DOIs
StatePublished - Jul 2004

Funding

FundersFunder number
National Cancer InstituteR37CA030488

    Fingerprint

    Dive into the research topics of 'RNA sequences in the Moloney murine leukemia virus genome bound by the Gag precursor protein in the yeast three-hybrid system'. Together they form a unique fingerprint.

    Cite this