Rhein, a selective inhibitor of the DPNH-flavin step in mitochondrial electron transport

E. A. Kean, M. Gutman, Thomas P. Singer

Research output: Contribution to journalArticlepeer-review

Abstract

Previous findings in the literature that rhein inhibits DPNH-linked mitochondrial oxidations by acting in the DPNH dehydrogenase region of the respiratory chain have been confirmed and extended. In the micromolar range rhein inhibits DPNH oxidase and DPNH-ferricyanide activities and the energy-linked reduction of DPN by succinate in membrane preparations from heart, as well as the DPNH dehydrogenase and transhydrogenase activities of the soluble, purified enzyme. The inhibition of the activities of the soluble enzyme are purely competitive with respect to substrate. These facts localize the primary inhibition site of rhein between substrate and FMN. In. heart ETP a second noncompetitive inhibition is also present but is detectable only at very low (<10 μM) rhein concentrations. Rhein also inhibits DPNH dehydrogenase in Candida utilis mitochondria and the purified enzyme from liver. On conversion of the heart enzyme to the low molecular weight DPNH-cytochrome reductase the typical effect of rhein disappears and is replaced by a slight stimulation or inhibition, depending on the electron acceptor used, showing that the substrate-binding site is modified in this form of the enzyme. In beef liver mitochondria DPNH oxidation may appear insensitive to rhein, probably because of the strong binding of rhein to other proteins. To a lesser extent unspecific binding of rhein and resultant interference with the inhibition of DPNH dehydrogenase is also shown by BSA and by proteins in heart ETP. Rhein also inhibits transhydrogenations in mitochondria and at higher concentrations lactate and malate dehydrogenases but has no effect on succinate, alcohol (liver and yeast), and glucose-6-P dehydrogenases or on Neurospora DPN-ase, glucose-6-phosphatase, and amine oxidase.

Original languageEnglish
Pages (from-to)1507-1513
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume40
Issue number6
DOIs
StatePublished - 30 Sep 1970
Externally publishedYes

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