Review

Yair Aharonowitz; Jürgen Bergmeyer; Jesus M. Cantoral; Gerald Cohen; Arnold L. Demain; Uwe Fink; Jim Kinghorn; Horst Kleinkauf; Andrew MacCabe; Harriet Palissa; Eva Pfeifer; Torsten Schwecke; Henk van Liempt; Hans von Döhren; Saul Wolfe; Jinyou Zhang

doi:10.1038/nbt0793-807

Review

Yair Aharonowitz, Jürgen Bergmeyer, Jesus M. Cantoral, Gerald Cohen, Arnold L. Demain, Uwe Fink, Jim Kinghorn, Horst Kleinkauf, Andrew MacCabe, Harriet Palissa, Eva Pfeifer, Torsten Schwecke, Henk van Liempt, Hans von Döhren^*, Saul Wolfe, Jinyou Zhang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

ACV Synthetase forms the tripeptide precursor of penicillins and cephalosporins from a-aminoadipate, cysteine, and valine. Catalytic sites for substrate carboxyl activation as adenylates, peptide bond formations, epimerization and release of the tripeptide-thioester are integrated in multifunctional enzymes of 405 to 425 kD. These have been characterized from several pro- and eukaryotic Iactam producers. Implications of these results for the thio-template mechanism of peptide formation are discussed, as well as the use of this multienzyme as a model system for enzymatic peptide synthesis.

Original language	English
Pages (from-to)	807-810
Number of pages	4
Journal	Bio/Technology
Volume	11
Issue number	7
DOIs	https://doi.org/10.1038/nbt0793-807
State	Published - Jul 1993
Externally published	Yes

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abstract = "ACV Synthetase forms the tripeptide precursor of penicillins and cephalosporins from a-aminoadipate, cysteine, and valine. Catalytic sites for substrate carboxyl activation as adenylates, peptide bond formations, epimerization and release of the tripeptide-thioester are integrated in multifunctional enzymes of 405 to 425 kD. These have been characterized from several pro- and eukaryotic Iactam producers. Implications of these results for the thio-template mechanism of peptide formation are discussed, as well as the use of this multienzyme as a model system for enzymatic peptide synthesis.",

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AU - Aharonowitz, Yair

AU - Bergmeyer, Jürgen

AU - Cantoral, Jesus M.

AU - Cohen, Gerald

AU - Demain, Arnold L.

AU - Fink, Uwe

AU - Kinghorn, Jim

AU - Kleinkauf, Horst

AU - MacCabe, Andrew

AU - Palissa, Harriet

AU - Pfeifer, Eva

AU - Schwecke, Torsten

AU - van Liempt, Henk

AU - von Döhren, Hans

AU - Wolfe, Saul

AU - Zhang, Jinyou

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Review

Abstract

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