Reversible blockade of the respiratory burst in human neutrophils by a cleavable cross-linking agent

I. Aviram, E. R. Simons, B. M. Babior

Research output: Contribution to journalArticlepeer-review

Abstract

Human neutrophils treated with low concentrations of the homobifunctional cross-linking reagents disuccinimidyl suberate and dithiobis(succinimidylpropionate) failed to generate superoxide in response to any of several stimuli, including phorbol myristate acetate, fMet-Leu-Phe, A23187, fluoride, and opsonized zymosan. The cross-linking reagent interfered with the activation of NADPH oxidase, but not with its activity. Cells treated with succinimidyl butyrate, a monovalent analog of the cross-linkers, underwent a normal respiratory burst. Cross-linking also inhibited degranulation, phagocytosis, and fluorescence responses of potential-sensitive dyes but had little effect on lactate production, sugar transport, the binding of fMet-Leu-Phe, or the activity of various enzymes in the cross-linked neutrophils. Most of the cellular function inhibited through the reaction of neutrophils with the cleavable cross-linker dithiobis(succinimidylpropionate) could be restored by reduction of the disulfide bonds of the cell-bound cross-linker with dithiothreitol.

Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalJournal of Biological Chemistry
Volume259
Issue number1
StatePublished - 1984

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