'Retroposon' insertion into the cellular oncogene c-myc in canine transmissible venereal tumor

N. Katzir, G. Rechavi, J. B. Cohen, T. Unger, F. Simoni, S. Segal, D. Givol

Research output: Contribution to journalArticlepeer-review

Abstract

We examined by Southern blotting the state of the cellular oncogene c-myc in the dog transmissible venereal tumor. The tumor DNA contains a 16.8-kilobase pair (kbp) rearranged c-myc fragment in addition to the normal 15-kbp and 7.5-kbp fragments. We compared the structure of the cloned rearranged c-myc (rc-myc) with that of a cloned normal c-myc and found that the rearrangement was due to the insertion of a 1.8-kbp DNA upstream to the first exon of c-myc. The inserted DNA is flanked by 10-base-pair direct repeats and contains a dA-rich tail, suggesting its origin from mRNA. Partial sequence of the inserted element showed 62% homology with the primate interdispersed Kpn I repetitive element. These results provide an example for the behavior of repetitive DNA sequences like the Kpn I family, as movable elements that can transpose nearby to oncogenes or other structural genes and perhaps affect their activity.

Original languageEnglish
Pages (from-to)1054-1058
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number4
DOIs
StatePublished - 1985
Externally publishedYes

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