TY - JOUR
T1 - Retinol binding protein 4
T2 - An adipokine associated with intra-amniotic infection/inflammation
AU - Vaisbuch, Edi
AU - Mazaki-Tovi, Shali
AU - Kusanovic, Juan Pedro
AU - Erez, Offer
AU - Than, Nandor Gabor
AU - Kim, Sun Kwon
AU - Dong, Zhong
AU - Gotsch, Francesca
AU - Mittal, Pooja
AU - Chaiworapongsa, Tinnakorn
AU - Pacora, Percy
AU - Yeo, Lami
AU - Hassan, Sonia S.
AU - Romero, Roberto
N1 - Funding Information:
This research was supported (in part) by the Perinatology Research Branch, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, DHHS.
PY - 2010/2
Y1 - 2010/2
N2 - Objective.Retinol binding protein 4 (RBP4), a specific carrier for retinol in the blood, is a novel adipokine that has been implicated in the pathophysiology of insulin resistance, and its gene expression has been associated with adipose tissue inflammation. Recently, proteomic profiling of amniotic fluid (AF) from women with preterm labor (PTL) revealed over-expression of RBP4 in those who delivered preterm. The aim of this study was to determine whether RBP4 is present in AF, and if its concentrations change with gestational age, in the presence of labor, and intra-amniotic infection/inflammation (IAI) in patients with spontaneous PTL. Study design.This cross-sectional study included pregnant women in the following groups: (1) mid-trimester (n=30); (2) term not in labor (n=31); (3) term in labor (n=30); (4) spontaneous PTL without IAI who delivered at term (n=60); (5) PTL without IAI who delivered preterm (n=64); and (6) PTL with IAI (n=56). RBP4 concentrations in AF were determined by ELISA. Non-parametric statistics were used for analyses. Results.(1) RBP4 was detected in all AF samples; (2) among patients with PTL, women with IAI had a higher median AF RBP4 concentration than those without IAI who delivered preterm (1268.9ng/ml, interquartile range (IQR) 900.3-1970.1 vs. 815.8ng/ml, IQR 592.41098.1; p<0.001) and at term (828.7ng/ml, IQR 499.7-1119.6; p<0.001); (3) the median AF RBP4 concentration was higher in women in the mid-trimester than in those at term not in labor (1861.1ng/ml, IQR 1486.2-2034.3 vs. 766.1ng/ml, IQR 608.5-1154.1; p<0.0001; (4) the median AF RBP4 concentration did not differ significantly between patients with PTL without IAI who delivered preterm and those who delivered at term (p=0.7); and (5) among women at term, the median AF RBP4 concentrations was not significantly different between those in labor and those not in labor (p=0.4). Conclusions.RBP4 is a physiologic constituent of the AF. Among patients with PTL, the median AF concentration of immunoreactive RBP4 is elevated in pregnancies complicated by IAI. These results suggest that RBP4 may participate in the host response against IAI.
AB - Objective.Retinol binding protein 4 (RBP4), a specific carrier for retinol in the blood, is a novel adipokine that has been implicated in the pathophysiology of insulin resistance, and its gene expression has been associated with adipose tissue inflammation. Recently, proteomic profiling of amniotic fluid (AF) from women with preterm labor (PTL) revealed over-expression of RBP4 in those who delivered preterm. The aim of this study was to determine whether RBP4 is present in AF, and if its concentrations change with gestational age, in the presence of labor, and intra-amniotic infection/inflammation (IAI) in patients with spontaneous PTL. Study design.This cross-sectional study included pregnant women in the following groups: (1) mid-trimester (n=30); (2) term not in labor (n=31); (3) term in labor (n=30); (4) spontaneous PTL without IAI who delivered at term (n=60); (5) PTL without IAI who delivered preterm (n=64); and (6) PTL with IAI (n=56). RBP4 concentrations in AF were determined by ELISA. Non-parametric statistics were used for analyses. Results.(1) RBP4 was detected in all AF samples; (2) among patients with PTL, women with IAI had a higher median AF RBP4 concentration than those without IAI who delivered preterm (1268.9ng/ml, interquartile range (IQR) 900.3-1970.1 vs. 815.8ng/ml, IQR 592.41098.1; p<0.001) and at term (828.7ng/ml, IQR 499.7-1119.6; p<0.001); (3) the median AF RBP4 concentration was higher in women in the mid-trimester than in those at term not in labor (1861.1ng/ml, IQR 1486.2-2034.3 vs. 766.1ng/ml, IQR 608.5-1154.1; p<0.0001; (4) the median AF RBP4 concentration did not differ significantly between patients with PTL without IAI who delivered preterm and those who delivered at term (p=0.7); and (5) among women at term, the median AF RBP4 concentrations was not significantly different between those in labor and those not in labor (p=0.4). Conclusions.RBP4 is a physiologic constituent of the AF. Among patients with PTL, the median AF concentration of immunoreactive RBP4 is elevated in pregnancies complicated by IAI. These results suggest that RBP4 may participate in the host response against IAI.
KW - Adipokines
KW - Chorioamnionitis
KW - Microbial invasion of the amniotic cavity
KW - Pregnancy
KW - Preterm delivery
KW - Preterm labor
KW - RBP4
UR - http://www.scopus.com/inward/record.url?scp=75449100963&partnerID=8YFLogxK
U2 - 10.3109/14767050902994739
DO - 10.3109/14767050902994739
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C2 - 19900011
AN - SCOPUS:75449100963
SN - 1476-7058
VL - 23
SP - 111
EP - 119
JO - Journal of Maternal-Fetal and Neonatal Medicine
JF - Journal of Maternal-Fetal and Neonatal Medicine
IS - 2
ER -