TY - JOUR
T1 - Resonant cavity imaging
T2 - A means toward high-throughput label-free protein detection
AU - Bergstein, David A.
AU - Özkumur, Emre
AU - Wu, Arthur C.
AU - Yalçin, Ayça
AU - Colson, Jeremy R.
AU - Needham, James W.
AU - Irani, Rostem J.
AU - Gershoni, Jonathan M.
AU - Goldberg, Bennett B.
AU - DeLisi, Charles
AU - Ruane, Michael F.
AU - Ünlü, M. Selim
N1 - Funding Information:
Manuscript received September 4, 2007; revised October 29, 2007. This work was supported in part by the National Institute of General and Medical Sciences under Grant NIH R21 GM 074872-02, in part by the U.S. Army Research Laboratory under Grant DAAD17-99-2-0070, and in part by the Boston University Office of Technology Development. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the U.S. Army Research Laboratory or the U.S. Government.
PY - 2008/1
Y1 - 2008/1
N2 - The resonant cavity imaging biosensor (RCIB) is an optical technique for detecting molecular binding interactions label free at many locations in parallel that employs an optical resonant cavity for high sensitivity. Near-infrared light centered at 1512.5 nm couples resonantly through a Fabry-Perot cavity constructed from dielectric reflectors (Si/SiO2 ), one of which serves as the binding surface. As the wavelength is swept using a tunable laser, a near-infrared digital camera monitors cavity transmittance at each pixel. A wavelength shift in the local resonant response of the optical cavity indicates binding. Positioning the sensing surface with respect to the standing wave pattern of the electric field within the cavity controls the sensitivity with which the presence of bound molecules is detected. Transmitted intensity at thousands of pixel locations is recorded simultaneously in a 10 s, 5 nm scan. An initial proof-of-principle setup has been constructed. A test sample was fabricated with 25, 100-μm wide square features, each with a different density of 1-μm square depressions etched 12 nm into the SiO 2 surface. The average depth of each etched region was found with 0.05 nm rms precision. In a second test, avidin, bound selectively to biotin conjugated bovine serum albumin, was detected.
AB - The resonant cavity imaging biosensor (RCIB) is an optical technique for detecting molecular binding interactions label free at many locations in parallel that employs an optical resonant cavity for high sensitivity. Near-infrared light centered at 1512.5 nm couples resonantly through a Fabry-Perot cavity constructed from dielectric reflectors (Si/SiO2 ), one of which serves as the binding surface. As the wavelength is swept using a tunable laser, a near-infrared digital camera monitors cavity transmittance at each pixel. A wavelength shift in the local resonant response of the optical cavity indicates binding. Positioning the sensing surface with respect to the standing wave pattern of the electric field within the cavity controls the sensitivity with which the presence of bound molecules is detected. Transmitted intensity at thousands of pixel locations is recorded simultaneously in a 10 s, 5 nm scan. An initial proof-of-principle setup has been constructed. A test sample was fabricated with 25, 100-μm wide square features, each with a different density of 1-μm square depressions etched 12 nm into the SiO 2 surface. The average depth of each etched region was found with 0.05 nm rms precision. In a second test, avidin, bound selectively to biotin conjugated bovine serum albumin, was detected.
KW - Biochemistry
KW - Chemical transducers
KW - Optical resonance
UR - http://www.scopus.com/inward/record.url?scp=39449100215&partnerID=8YFLogxK
U2 - 10.1109/JSTQE.2007.913397
DO - 10.1109/JSTQE.2007.913397
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AN - SCOPUS:39449100215
SN - 1077-260X
VL - 14
SP - 131
EP - 138
JO - IEEE Journal of Selected Topics in Quantum Electronics
JF - IEEE Journal of Selected Topics in Quantum Electronics
IS - 1
ER -