Repression of the MSP/MST-1 gene contributes to the antiapoptotic gain of function of mutant p53

A. Zalcenstein, L. Weisz, P. Stambolsky, J. Bar, V. Rotter, M. Oren*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor-associated mutant forms of p53 can exert an antiapoptotic gain of function activity, which confers a selective advantage upon tumor cells harboring such mutations. We report that mutant p53 suppresses the expression of the MSP (MST-1/HGFL) gene, encoding the ligand of the receptor tyrosine kinase RON, implicated in a variety of cellular responses. Mutant p53 associates with the MSP gene promoter and represses its transcriptional activity, leading to a decrease in mRNA levels and a subsequent decrease in the levels of secreted MSP protein. Forced downregulation of MSP expression in H1299 cells, derived from a large-cell lung carcinoma, confers increased resistance against etoposide-induced cell death. These antiapoptotic consequences of MSP down-regulation seemingly conflict with the well-documented ability of the RON receptor to promote cell survival and tumor progression when aberrantly hyperactive. Yet, they are consistent with the fact that reduced MSP expression was observed in many types of human cancer, including large-cell lung carcinoma. Thus, repression of MSP gene expression by mutant p53 may contribute to oncogenesis in a cell type-specific manner.

Original languageEnglish
Pages (from-to)359-369
Number of pages11
JournalOncogene
Volume25
Issue number3
DOIs
StatePublished - 19 Jan 2006
Externally publishedYes

Funding

FundersFunder number
EC FP6R37 CA40099, 502983
Yad Abraham Center for Cancer Diagnosis and Therapy
National Cancer InstituteR37CA040099
Flight Attendant Medical Research Institute

    Keywords

    • Apoptosis
    • MSP
    • MST-1
    • RON
    • p53

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