TY - JOUR
T1 - Replication pattern in cancer
T2 - Asynchronous replication in multiple myeloma and in monoclonal gammopathy
AU - Amiel, A.
AU - Kirgner, I.
AU - Gaber, E.
AU - Manor, Y.
AU - Fejgin, M.
AU - Lishner, M.
PY - 1999/1
Y1 - 1999/1
N2 - In this study we evaluated the replication pattern and cell-cycle dynamics of cells from patients considered to have a premalignant condition (monoclonal gammopathy, or MGUS) and patients with multiple myeloma (MM), as well as healthy controls. We applied the fluorescence in situ hybridization (FISH) technique with the TP53, RB-1 and 21q22 loci on the patient's cells. Asynchrony was determined by the presence of one single and one set of double dots in the same cell. The rate of asynchronic replication was significantly higher in the cells from MM patients, with intermediate value in the cells from MGUS, while the lowest rate was in cells from controls. We suggest that these results may reflect the changes in gene replication and cell-cycle progression that occur in premalignant and malignant cells.
AB - In this study we evaluated the replication pattern and cell-cycle dynamics of cells from patients considered to have a premalignant condition (monoclonal gammopathy, or MGUS) and patients with multiple myeloma (MM), as well as healthy controls. We applied the fluorescence in situ hybridization (FISH) technique with the TP53, RB-1 and 21q22 loci on the patient's cells. Asynchrony was determined by the presence of one single and one set of double dots in the same cell. The rate of asynchronic replication was significantly higher in the cells from MM patients, with intermediate value in the cells from MGUS, while the lowest rate was in cells from controls. We suggest that these results may reflect the changes in gene replication and cell-cycle progression that occur in premalignant and malignant cells.
UR - http://www.scopus.com/inward/record.url?scp=0032907575&partnerID=8YFLogxK
U2 - 10.1016/S0165-4608(98)00107-1
DO - 10.1016/S0165-4608(98)00107-1
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AN - SCOPUS:0032907575
SN - 0165-4608
VL - 108
SP - 32
EP - 37
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -
