Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

Shirly Berezin; Yaron Aviv; Hagit Aviv; Elad Goldberg; Yaakov R. Tischler

doi:10.1038/s41598-017-02212-2

Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

Shirly Berezin, Yaron Aviv, Hagit Aviv, Elad Goldberg, Yaakov R. Tischler^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Rapid and accurate Gram differentiation is paramount as the first step of pathogen identification and antibiotics administration. However, the current method requires additional reagents, is time-consuming, and is operator dependent. Here we show the principle of tip enhanced Raman spectroscopy (TERS) can differentiate between Gram negative and positive species, by detecting the changes in tip-enhancement in the Raman scattering from the bacteria's lipid-bilayer membrane, which specifically enhances Gram negative bacteria.

Original language	English
Article number	3810
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-02212-2
State	Published - 1 Dec 2017
Externally published	Yes

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Replacing a Century Old Technique-Modern Spectroscopy Can Supplant Gram Staining

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