TY - JOUR
T1 - Repair of x-ray-induced single strand breaks in toluenized Escherichia coli cells
AU - Waldstein, Evelyn A.
AU - Setlow, R. B.
N1 - Funding Information:
This investigation was sponsored by the Jewish Agency through the Israeli Center of Absorption in Science. We are indebted to R. Ben-lshai for supplying bacterial strains and S. Manoilis for perforrning some of the experiments. This work was carried out, in part, at Brookhaven National Laboratory under the auspices of the U.S. Energy Research and Develol~ment Administration.
PY - 1976/8/18
Y1 - 1976/8/18
N2 - We have used sedimentation in alkali to estimate the repair of X-ray-induced single strand breaks in the DNA of irradiated toluenized Escherichia coli cells. Extensive repair requires no exogenous cofactors except ATP although other individual NTPs (except U) or dNTPs can substitute for ATP. There is no repair in polA or resA cells and since nicotinamide mononucleotide (NMN) inhibits repair in wild type cells we interpret the results as indicating that both ligase and polymerase I are needed for repair but that the amount of any gap filling is small and extensive repair replication is not necessary.
AB - We have used sedimentation in alkali to estimate the repair of X-ray-induced single strand breaks in the DNA of irradiated toluenized Escherichia coli cells. Extensive repair requires no exogenous cofactors except ATP although other individual NTPs (except U) or dNTPs can substitute for ATP. There is no repair in polA or resA cells and since nicotinamide mononucleotide (NMN) inhibits repair in wild type cells we interpret the results as indicating that both ligase and polymerase I are needed for repair but that the amount of any gap filling is small and extensive repair replication is not necessary.
UR - http://www.scopus.com/inward/record.url?scp=0017177910&partnerID=8YFLogxK
U2 - 10.1016/0005-2787(76)90486-X
DO - 10.1016/0005-2787(76)90486-X
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 182255
AN - SCOPUS:0017177910
SN - 0005-2787
VL - 442
SP - 154
EP - 161
JO - BBA Section Nucleic Acids And Protein Synthesis
JF - BBA Section Nucleic Acids And Protein Synthesis
IS - 2
ER -