TY - JOUR
T1 - Renewable fatty acid ester production in Clostridium
AU - Feng, Jun
AU - Zhang, Jie
AU - Ma, Yuechao
AU - Feng, Yiming
AU - Wang, Shangjun
AU - Guo, Na
AU - Wang, Haijiao
AU - Wang, Pixiang
AU - Jiménez-Bonilla, Pablo
AU - Gu, Yanyan
AU - Zhou, Junping
AU - Zhang, Zhong Tian
AU - Cao, Mingfeng
AU - Jiang, Di
AU - Wang, Shuning
AU - Liu, Xian Wei
AU - Shao, Zengyi
AU - Borovok, Ilya
AU - Huang, Haibo
AU - Wang, Yi
N1 - Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3 g/L butyl acetate and 1.6 g/L butyl butyrate. Techno-economic analysis results suggest the economic competitiveness of our developed bioprocess. Our principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable end products may be applicable to other bioprocesses.
AB - Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3 g/L butyl acetate and 1.6 g/L butyl butyrate. Techno-economic analysis results suggest the economic competitiveness of our developed bioprocess. Our principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable end products may be applicable to other bioprocesses.
UR - http://www.scopus.com/inward/record.url?scp=85110758558&partnerID=8YFLogxK
U2 - 10.1038/s41467-021-24038-3
DO - 10.1038/s41467-021-24038-3
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C2 - 34272383
AN - SCOPUS:85110758558
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 4368
ER -