TY - JOUR
T1 - Reliable determination of transposon insertion site in prokaryotes by direct sequencing
AU - Qimron, Udi
AU - Madar, Neta
AU - Ascarelli-Goell, Rivi
AU - Elgrably-Weiss, Maya
AU - Altuvia, Shoshy
AU - Porgador, Angel
N1 - Funding Information:
This study was supported by grants from the USA–Israel Binational Science Foundation, Israel Cancer Research Foundation and Israel Cancer Association. This work eas also supported by the Cooperation Program of the Deutsches Krebsforschungszentrum (DKFZ) and Israel's Ministry of Science (MOS). U.Q. was supported by the Kreitman Foundation in BGU.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a transposon internal primer. Reliable direct sequencing was achieved using high purity genomic DNA and an improved protocol for automated sequence machine. This note is intended to promote the use of direct sequencing, which we found to be reliable, efficient and inexpensive as compared to the other currently used methods.
AB - We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a transposon internal primer. Reliable direct sequencing was achieved using high purity genomic DNA and an improved protocol for automated sequence machine. This note is intended to promote the use of direct sequencing, which we found to be reliable, efficient and inexpensive as compared to the other currently used methods.
KW - Direct genomic sequencing
KW - Transposon
KW - Transposon insertion site
UR - http://www.scopus.com/inward/record.url?scp=0038742654&partnerID=8YFLogxK
U2 - 10.1016/S0167-7012(03)00033-2
DO - 10.1016/S0167-7012(03)00033-2
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AN - SCOPUS:0038742654
SN - 0167-7012
VL - 54
SP - 137
EP - 140
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 1
ER -