Reliable determination of transposon insertion site in prokaryotes by direct sequencing

Udi Qimron, Neta Madar, Rivi Ascarelli-Goell, Maya Elgrably-Weiss, Shoshy Altuvia, Angel Porgador*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a transposon internal primer. Reliable direct sequencing was achieved using high purity genomic DNA and an improved protocol for automated sequence machine. This note is intended to promote the use of direct sequencing, which we found to be reliable, efficient and inexpensive as compared to the other currently used methods.

Original languageEnglish
Pages (from-to)137-140
Number of pages4
JournalJournal of Microbiological Methods
Volume54
Issue number1
DOIs
StatePublished - 1 Jul 2003
Externally publishedYes

Funding

FundersFunder number
Cooperation Program of the Deutsches Krebsforschungszentrum
Israel's Ministry of Science
Kreitman Foundation in BGU
Israel Cancer Research Fund
Maryland Ornithological Society
German Cancer Research Center
United States-Israel Binational Science Foundation
Israel Cancer Association

    Keywords

    • Direct genomic sequencing
    • Transposon
    • Transposon insertion site

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