TY - JOUR
T1 - Release of atrial natriuretic peptide in brief ischemia-reperfusion in isolated rat hearts
AU - Arad, M.
AU - Zamir, N.
AU - Horowitz, L.
AU - Oxman, T.
AU - Rabinowitz, B.
PY - 1994
Y1 - 1994
N2 - We studied the effects of short-term global ischemia and reperfusion on ANP secretion from Langendorff-perfused rat hearts compared with isolated ventricles. Effects of regional ischemia, with or without increased atrial pressure, were examined in Langendorff-perfused and working heart models. Five minutes of global ischemia were associated with elevated levels of atrial natriuretic peptide (ANP) in the coronary effluent immediately and for ≃10 min after resumption of reperfusion, resulting in a net hormone excess of 23 ± 5 ng/g wet wt. The ventricles produced on the average 11% ANP compared with the whole heart, and their contribution of to postischemic ANP overflow was approximately proportional to their basal production. In Langendorff-perfused hearts, regional ischemia increased the concentration of ANP in the coronary effluent 51 ± 11%, whereas the secretion rate (per minute) decreased 18 ± 5%. In the presence of atrial distension in the working heart model, a trend for increase in ANP secretion was apparent. We conclude that global ischemia, even of brief duration, has an independent stimulatory effect on ANP release, the ischemic atrium being responsible for most of the excess. Regional ischemia, when not accompanied by atrial distension, reduces the ANP secretion rate during the ischemic period. Heart failure secondary to ischemia stimulates ANP secretion, but this response seems to be both delayed and attenuated compared with atrial stretch alone.
AB - We studied the effects of short-term global ischemia and reperfusion on ANP secretion from Langendorff-perfused rat hearts compared with isolated ventricles. Effects of regional ischemia, with or without increased atrial pressure, were examined in Langendorff-perfused and working heart models. Five minutes of global ischemia were associated with elevated levels of atrial natriuretic peptide (ANP) in the coronary effluent immediately and for ≃10 min after resumption of reperfusion, resulting in a net hormone excess of 23 ± 5 ng/g wet wt. The ventricles produced on the average 11% ANP compared with the whole heart, and their contribution of to postischemic ANP overflow was approximately proportional to their basal production. In Langendorff-perfused hearts, regional ischemia increased the concentration of ANP in the coronary effluent 51 ± 11%, whereas the secretion rate (per minute) decreased 18 ± 5%. In the presence of atrial distension in the working heart model, a trend for increase in ANP secretion was apparent. We conclude that global ischemia, even of brief duration, has an independent stimulatory effect on ANP release, the ischemic atrium being responsible for most of the excess. Regional ischemia, when not accompanied by atrial distension, reduces the ANP secretion rate during the ischemic period. Heart failure secondary to ischemia stimulates ANP secretion, but this response seems to be both delayed and attenuated compared with atrial stretch alone.
KW - Langendorff model
KW - global ischemia
KW - left atrial pressure
KW - regional ischemia
KW - working heart
UR - http://www.scopus.com/inward/record.url?scp=0028028862&partnerID=8YFLogxK
U2 - 10.1152/ajpheart.1994.266.5.h1971
DO - 10.1152/ajpheart.1994.266.5.h1971
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AN - SCOPUS:0028028862
SN - 0363-6135
VL - 266
SP - H1971-H1978
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 5 35-5
ER -