TY - JOUR
T1 - Release of a small two-chain form of antithrombin III from a conformationally changed antithrombin III-thrombin complex
AU - Knoller, Sarah
AU - Savion, Naphtali
N1 - Funding Information:
This work was supported by a grant from the National Council for Research and Development of Israel and the Gesellschaft fur Strahlen und Umwelt Forschung mbH (GSF), Munich, Federal Republic of Germany.
PY - 1991/7/15
Y1 - 1991/7/15
N2 - Reaction of antithrombin III (AT) with thrombin results in the formation of stable antithrombin III-thrombin (AT-T) complex with a Mr of 92.5-kDa, accompanied by the appearance of a proteolytically modified form of the inhibitor (ATM). Under these conditions AT-T is also transformed to a smaller complex (AT-TS). This smaller complex (81-kDa), a product of a conformational change at the AT moiety of the AT-T complex, is further transformed to a very small complex (AT-TVS) with a Mr of 71-kDa. Along with this process, AT-TS slowly dissociates to a free enzyme and a small, presumably to-chain product of AT (ATMS) with a Mr of 49-kDa. The newly described component, ATMS, naturally occurs in plasma and serum and accumulates significantly in plasma of patients suffering from cardiovascular disease.
AB - Reaction of antithrombin III (AT) with thrombin results in the formation of stable antithrombin III-thrombin (AT-T) complex with a Mr of 92.5-kDa, accompanied by the appearance of a proteolytically modified form of the inhibitor (ATM). Under these conditions AT-T is also transformed to a smaller complex (AT-TS). This smaller complex (81-kDa), a product of a conformational change at the AT moiety of the AT-T complex, is further transformed to a very small complex (AT-TVS) with a Mr of 71-kDa. Along with this process, AT-TS slowly dissociates to a free enzyme and a small, presumably to-chain product of AT (ATMS) with a Mr of 49-kDa. The newly described component, ATMS, naturally occurs in plasma and serum and accumulates significantly in plasma of patients suffering from cardiovascular disease.
KW - Antithrombin III-Thrombin complex
KW - Thrombin modified Antithrombin III
UR - http://www.scopus.com/inward/record.url?scp=0025734343&partnerID=8YFLogxK
U2 - 10.1016/0049-3848(91)90284-4
DO - 10.1016/0049-3848(91)90284-4
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AN - SCOPUS:0025734343
SN - 0049-3848
VL - 63
SP - 203
EP - 214
JO - Thrombosis Research
JF - Thrombosis Research
IS - 2
ER -
