Release from apoptosis correlates with tumor progression in the AKR lymphoma

Sigalit Kay, Natalie Donin, Moshe Michowitz, Dina Katzenelson, Jehuda Hiss, Ginnette Schibi, Asher Pinchassov, Judith Leibovici*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Disturbance of apoptosis is an established factor in tumorigenesis. The role of apoptosis in tumor progression is not yet clear. In the present study we compared the tendency to spontaneous apoptosis (and the proliferative capacity) of tumor cells derived from primary (PT) and metastatic tumor (MT) cells of several AKR lymphoma variants. Apoptosis-related gene expression was also compared. Our results indicate that release from apoptosis has a role in the tumor progression of this T cell lymphoma. At the cellular level, a markedly lower apoptotic tendency was observed in MT than in PT cells. The existence of macrophages only in PT also supports the presence of apoptotic cells in local but not in MTs. By contrast, proliferative capacity does not determine tumor aggressiveness in this system. At the molecular level, we found a higher staining intensity for bcl-2 in MT than in PT cells, suggesting that bcl-2 might be responsible for the reduced apoptosis in MT compared to PT cells. Evidence for p53 overexpression was found in the MT cells of one of the variants but in none of the PT. Comparison of Fas receptor, unexpectedly showed an increased expression in MT versus PT cells, possibly indicating resistance to Fas-induced apoptosis in the MT cells. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)37-50
Number of pages14
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1497
Issue number1
DOIs
StatePublished - 2 Jun 2000

Keywords

  • AKR lymphoma
  • Apoptosis resistance
  • Apoptosis-related gene expression
  • Metastatic phenotype
  • Primary versus metastatic tumor
  • Tumor progression

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