Regulation of rat brain vesicular monoamine transporter by chronic treatment with ovarian hormones

Moshe Rehavi*, Miri Goldin, Netta Roz, Abraham Weizman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Ovarian steroids play an important role in neuroregulation and in the pathophysiology of various neuropsychiatric disorders. Most of the studies focused on the impact of gonadal steroids on post-synaptic receptors and plasma membrane transporters. In the present study, we evaluated the effect of chronic treatment with ovarian steroids on the expression of rat brain vesicular monoamine transporter (VMAT2). Ovariectomized rats were treated for 21 days with estradiol, progesterone or both. VMAT2 gene expression was assessed on the protein level by high affinity [3H]dihydrotetrabenazine ([3H]TBZOH) binding using autoradiography and on the mRNA level by in situ hybridization. Progesterone administration led to a decrease in [3H]TBZOH binding in the middle striatum and in the nucleus accumbens and to a parallel decrease in VMAT2 mRNA level in the substantia nigra pars compacta and dorsal raphe nuclei. Chronic estradiol treatment reduced VMAT2 mRNA level in the dorsal raphe and [3H]TBZOH binding in middle part of the striatum and nucleus accumbens but did not affect VMAT2 mRNA level in the substantia nigra pars compacta. Simultaneous administration of both ovarian steroids did not modulate VMAT2 mRNA in the substantia nigra pars compacta as well as [3H]TBZOH binding in the striatum or the nucleus accumbens but reduced VMAT2 mRNA level in the dorsal raphe. It appears that ovarian steroids may play a crucial role in the regulation of VMAT2 gene expression in the dopamine and serotonin systems. This modulatory activity may be relevant to synaptic and neuronal plasticity as well as to the molecular and cellular pathophysiology of gender-specific neuropsychiatric disorders.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalMolecular Brain Research
Volume57
Issue number1
DOIs
StatePublished - 1 Jun 1998

Keywords

  • Dopamine
  • Estradiol
  • Progesterone
  • Serotonin
  • Vesicular monoamine transporter
  • [H]dihydrotetrabenazine

Fingerprint

Dive into the research topics of 'Regulation of rat brain vesicular monoamine transporter by chronic treatment with ovarian hormones'. Together they form a unique fingerprint.

Cite this