Regulation of glucose dynamics by noninvasive peripheral electrical stimulation in normal and insulin-resistant rats

Merav Catalogna*, Sigal Fishman, Zamir Halpern, Shani Ben-Shlomo, Uri Nevo, Eshel Ben-Jacob

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Background The epidemic nature of type 2 diabetes mellitus (T2DM), along with the downsides of current treatments, has raised the need for therapeutic alternatives. Methods We studied normo-glycemic and high-fat diet (HFD), induced insulin-resistant Wistar Han rats for 2 to 3 weeks. Rats received peripheral electrical stimulation (PES) treatment (2 Hz/16 Hz bursts, 10 mA) in their hind limbs for 3 min, 3 times per week. Glucose tolerance was evaluated by using a glucose tolerance test at the beginning and again at the end of the study. The effect of an acute PES treatment on metabolic rates of glucose appearance and turnover was measured by using the hyperinsulinemic-euglycemic clamp (HEGC) test. Results Repeated PES treatment significantly inhibited the progression of glucose intolerance in normal and insulin-resistant rats and prevented HFD-induced gains in body weight and fat mass. Acute treatment induced a prolonged effect on glucose turnover, as evaluated by the HEGC test. Increased hepatic glucose output was observed during the basal state (P < 0.005). Under hyperinsulinemic conditions, PES improved tissue sensitivity to insulin (41.1%, P < 0.01), improved suppression of hepatic glucose production (58.9 ± 4.4% vs. 87.1 ± 4.4%, P < 0.02) and significantly elevated the rate of glycogenesis (P < 0.01), compared with controls. Conclusions The present study indicates that a noninvasive PES treatment of very short duration is sufficiently potent to stimulate glucose utilization and improve hepatic insulin sensitivity in rats. Repeated PES treatment may have a beneficial effect on HFD-induced adiposity and control of body weight.

Original languageEnglish
Pages (from-to)863-873
Number of pages11
JournalMetabolism: Clinical and Experimental
Issue number6
StatePublished - Jun 2016


FundersFunder number
Tauber Family Foundation
National Science Foundation1427654
Tel Aviv University


    • Glucose clamp test
    • Glycogenesis
    • Hepatic insulin sensitivity
    • Insulin resistance
    • Noninvasive electrical stimulation


    Dive into the research topics of 'Regulation of glucose dynamics by noninvasive peripheral electrical stimulation in normal and insulin-resistant rats'. Together they form a unique fingerprint.

    Cite this