@article{1b4eff187633459e8e72e066fe1a73a2,
title = "Regulation of a major cloned voltage-gated K+ channel from human T lymphocytes",
abstract = "When expressed into Xenopus oocytes, HLK3 K+ channel (Kvl-3) induced a slowly inactivating voltage-dependent K+ current. We have studied the modulation of this K+ current by co-expressing a cloned 5-HT2 receptor together with HLK3K+ channel protein. Application of 5-HT caused a long-lasting inhibition of the voltage-gated K+ current. This inhibitory modulation was mimicked by intracellular injection of inositol triphosphate or Ca2+, as well as by incubation with phorbol esters or diacylglycerol analogs. Oocytes pretreatment with staurosporine and EGTA fully prevented 5-HT inhibitory action. Elevation of cAMP and cGMP levels into oocytes did not produce any detectable effect on the current recorded in the absence or the presence of 5-HT. These data suggest that the second messengers generated by phospholipase C activation may be important modulators of HLK3 K+ channels in the immune and the central nervous systems.",
keywords = "Calcium, Jurkat, Kinase C, Serotonin, T cell",
author = "Bernard Attali and Eric Honor{\'e} and Florian Lesage and Michael Lazdunski and Jacques Barhanin",
note = "Funding Information: ing for providin~ the 5-HT, reeepter clone. We thank M.-M. Larro-qu{\textcopyright}, C. Roulinat and F, Aguila for skillful technical assistance. B.A. is a recipient of a fellowshipf rom the Association pour la Recherche contr¢ le Cancer. This work was supported by the Centre National de la Recherche Seientifique, the Association pour la Recherche eontre le Cancer and the Association pour la Recherche sur la S¢l~rose en Plaques,",
year = "1992",
month = jun,
day = "1",
doi = "10.1016/0014-5793(92)80526-M",
language = "אנגלית",
volume = "303",
pages = "229--232",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "2-3",
}