Regulation of a major cloned voltage-gated K+ channel from human T lymphocytes

Bernard Attali; Eric Honoré; Florian Lesage; Michael Lazdunski; Jacques Barhanin

doi:10.1016/0014-5793(92)80526-M

Regulation of a major cloned voltage-gated K⁺ channel from human T lymphocytes

Bernard Attali, Eric Honoré^*, Florian Lesage, Michael Lazdunski, Jacques Barhanin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

When expressed into Xenopus oocytes, HLK₃ K⁺ channel (Kvl-3) induced a slowly inactivating voltage-dependent K⁺ current. We have studied the modulation of this K⁺ current by co-expressing a cloned 5-HT₂ receptor together with HLK₃K⁺ channel protein. Application of 5-HT caused a long-lasting inhibition of the voltage-gated K⁺ current. This inhibitory modulation was mimicked by intracellular injection of inositol triphosphate or Ca²⁺, as well as by incubation with phorbol esters or diacylglycerol analogs. Oocytes pretreatment with staurosporine and EGTA fully prevented 5-HT inhibitory action. Elevation of cAMP and cGMP levels into oocytes did not produce any detectable effect on the current recorded in the absence or the presence of 5-HT. These data suggest that the second messengers generated by phospholipase C activation may be important modulators of HLK₃ K⁺ channels in the immune and the central nervous systems.

Original language	English
Pages (from-to)	229-232
Number of pages	4
Journal	FEBS Letters
Volume	303
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(92)80526-M
State	Published - 1 Jun 1992
Externally published	Yes

Keywords

Calcium
Jurkat
Kinase C
Serotonin
T cell

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10.1016/0014-5793(92)80526-M

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title = "Regulation of a major cloned voltage-gated K+ channel from human T lymphocytes",

abstract = "When expressed into Xenopus oocytes, HLK3 K+ channel (Kvl-3) induced a slowly inactivating voltage-dependent K+ current. We have studied the modulation of this K+ current by co-expressing a cloned 5-HT2 receptor together with HLK3K+ channel protein. Application of 5-HT caused a long-lasting inhibition of the voltage-gated K+ current. This inhibitory modulation was mimicked by intracellular injection of inositol triphosphate or Ca2+, as well as by incubation with phorbol esters or diacylglycerol analogs. Oocytes pretreatment with staurosporine and EGTA fully prevented 5-HT inhibitory action. Elevation of cAMP and cGMP levels into oocytes did not produce any detectable effect on the current recorded in the absence or the presence of 5-HT. These data suggest that the second messengers generated by phospholipase C activation may be important modulators of HLK3 K+ channels in the immune and the central nervous systems.",

keywords = "Calcium, Jurkat, Kinase C, Serotonin, T cell",

author = "Bernard Attali and Eric Honor{\'e} and Florian Lesage and Michael Lazdunski and Jacques Barhanin",

note = "Funding Information: ing for providin~ the 5-HT, reeepter clone. We thank M.-M. Larro-qu{\textcopyright}, C. Roulinat and F, Aguila for skillful technical assistance. B.A. is a recipient of a fellowshipf rom the Association pour la Recherche contr¢ le Cancer. This work was supported by the Centre National de la Recherche Seientifique, the Association pour la Recherche eontre le Cancer and the Association pour la Recherche sur la S¢l~rose en Plaques,",

year = "1992",

month = jun,

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doi = "10.1016/0014-5793(92)80526-M",

language = "אנגלית",

volume = "303",

pages = "229--232",

journal = "FEBS Letters",

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T1 - Regulation of a major cloned voltage-gated K+ channel from human T lymphocytes

AU - Attali, Bernard

AU - Honoré, Eric

AU - Lesage, Florian

AU - Lazdunski, Michael

AU - Barhanin, Jacques

N1 - Funding Information: ing for providin~ the 5-HT, reeepter clone. We thank M.-M. Larro-qu©, C. Roulinat and F, Aguila for skillful technical assistance. B.A. is a recipient of a fellowshipf rom the Association pour la Recherche contr¢ le Cancer. This work was supported by the Centre National de la Recherche Seientifique, the Association pour la Recherche eontre le Cancer and the Association pour la Recherche sur la S¢l~rose en Plaques,

PY - 1992/6/1

Y1 - 1992/6/1

N2 - When expressed into Xenopus oocytes, HLK3 K+ channel (Kvl-3) induced a slowly inactivating voltage-dependent K+ current. We have studied the modulation of this K+ current by co-expressing a cloned 5-HT2 receptor together with HLK3K+ channel protein. Application of 5-HT caused a long-lasting inhibition of the voltage-gated K+ current. This inhibitory modulation was mimicked by intracellular injection of inositol triphosphate or Ca2+, as well as by incubation with phorbol esters or diacylglycerol analogs. Oocytes pretreatment with staurosporine and EGTA fully prevented 5-HT inhibitory action. Elevation of cAMP and cGMP levels into oocytes did not produce any detectable effect on the current recorded in the absence or the presence of 5-HT. These data suggest that the second messengers generated by phospholipase C activation may be important modulators of HLK3 K+ channels in the immune and the central nervous systems.

AB - When expressed into Xenopus oocytes, HLK3 K+ channel (Kvl-3) induced a slowly inactivating voltage-dependent K+ current. We have studied the modulation of this K+ current by co-expressing a cloned 5-HT2 receptor together with HLK3K+ channel protein. Application of 5-HT caused a long-lasting inhibition of the voltage-gated K+ current. This inhibitory modulation was mimicked by intracellular injection of inositol triphosphate or Ca2+, as well as by incubation with phorbol esters or diacylglycerol analogs. Oocytes pretreatment with staurosporine and EGTA fully prevented 5-HT inhibitory action. Elevation of cAMP and cGMP levels into oocytes did not produce any detectable effect on the current recorded in the absence or the presence of 5-HT. These data suggest that the second messengers generated by phospholipase C activation may be important modulators of HLK3 K+ channels in the immune and the central nervous systems.

KW - Calcium

KW - Jurkat

KW - Kinase C

KW - Serotonin

KW - T cell

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U2 - 10.1016/0014-5793(92)80526-M

DO - 10.1016/0014-5793(92)80526-M

M3 - מאמר

AN - SCOPUS:0026548095

VL - 303

SP - 229

EP - 232

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2-3

ER -

Regulation of a major cloned voltage-gated K⁺ channel from human T lymphocytes

Abstract

Keywords

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