Regional heterogeneity of two high affinity binding sites for 3H-WB-4101 in mouse brain

Moshe Rehavi*, Bella Yavetz, Orly Ramot, Mordechai Sokolovsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Binding studies of the tritium labeled α-adrenergic antagonist WB-4101 to mouse brain homogenate at equilibrium, reveal two binding sites: a super high affinity site (0.25 nM, 0.070 pmole/mg. protein) and a high affinity site (3.0 nM, 0.19 pmole/ mg. protein). Kinetic experiments, which measure the rates of association and dissociation of 3H-WB-4101 further confirm the existence of two binding sites. Differences in the distribution of the two sites in five regions of mouse brain are described. In addition, we present evidence suggestive of a postsynaptic localization of both binding sites.

Original languageEnglish
Pages (from-to)615-621
Number of pages7
JournalLife Sciences
Volume26
Issue number8
DOIs
StatePublished - 25 Feb 1980

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