TY - JOUR
T1 - Regional heterogeneity of two high affinity binding sites for 3H-WB-4101 in mouse brain
AU - Rehavi, Moshe
AU - Yavetz, Bella
AU - Ramot, Orly
AU - Sokolovsky, Mordechai
PY - 1980/2/25
Y1 - 1980/2/25
N2 - Binding studies of the tritium labeled α-adrenergic antagonist WB-4101 to mouse brain homogenate at equilibrium, reveal two binding sites: a super high affinity site (0.25 nM, 0.070 pmole/mg. protein) and a high affinity site (3.0 nM, 0.19 pmole/ mg. protein). Kinetic experiments, which measure the rates of association and dissociation of 3H-WB-4101 further confirm the existence of two binding sites. Differences in the distribution of the two sites in five regions of mouse brain are described. In addition, we present evidence suggestive of a postsynaptic localization of both binding sites.
AB - Binding studies of the tritium labeled α-adrenergic antagonist WB-4101 to mouse brain homogenate at equilibrium, reveal two binding sites: a super high affinity site (0.25 nM, 0.070 pmole/mg. protein) and a high affinity site (3.0 nM, 0.19 pmole/ mg. protein). Kinetic experiments, which measure the rates of association and dissociation of 3H-WB-4101 further confirm the existence of two binding sites. Differences in the distribution of the two sites in five regions of mouse brain are described. In addition, we present evidence suggestive of a postsynaptic localization of both binding sites.
UR - http://www.scopus.com/inward/record.url?scp=0018901531&partnerID=8YFLogxK
U2 - 10.1016/0024-3205(80)90237-4
DO - 10.1016/0024-3205(80)90237-4
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AN - SCOPUS:0018901531
SN - 0024-3205
VL - 26
SP - 615
EP - 621
JO - Life Sciences
JF - Life Sciences
IS - 8
ER -