Reduction of β-amyloid plaques in brain of transgenic mouse model of Alzheimer's disease by EFRH-phage immunization

Dan Frenkel, Ilse Dewachter, Fred Van Leuven, Beka Solomon

Research output: Contribution to journalArticlepeer-review

Abstract

Antibodies to the epitope EFRH, representing residues 3-6 within the β-amyloid (Aβ) sequence, were previously shown to affect the solubility and disaggregation of Aβ fibrils in vitro. Here, we describe a novel method of immunization, using as antigen the EFRH peptide displayed on the surface of the filamentous phage. The EFRH phage evoked effective auto-immune antibodies in amyloid precursor protein [V717I] (APP[V717I]) transgenic mice that recapitulate the amyloid plaques and vascular pathology of Alzheimer's disease (AD). The immunization provoked a considerable reduction in the number of Aβ amyloid plaques in the brain of the transgenic mice and may serve as the basis for anti-Aβ vaccine.

Original languageEnglish
Pages (from-to)1060-1065
Number of pages6
JournalVaccine
Volume21
Issue number11-12
DOIs
StatePublished - 7 Mar 2003

Keywords

  • APP-transgenic mice
  • Alzheimer's plaque clearance
  • EFRH-epitope

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