The aim of this study was to compare the sialic acid content in chronic lymphocytic leukemia (CLL) lymphocytes to that of normal lymphocytes and to find out whether alterations eventually observed could be correlated with the activity of neuraminidase in serum of CLL patients. Sialic acid in lymphocytes was determined after release by hydrolysis for 1 h with 1N HCl at 80°C. Neuraminidase activity in serum was determined by measuring the sialic acid released after incubation with N-acetylneuramin-lactose as substrate. The results showed that CLL lymphocytes contained less sialic acid than normal lymphocytes, the difference being highly significant (120.1 ± 30.0 vs 44.9 ± 10.1 nmoles/108 cells). In our assay for neuraminidase activity in serum incubation with substrate for periods of up to 24 h failed to release detectable amounts of sialic acid by both leukemic and normal sera, thus precluding the possibility that this enzyme is responsible for the low levels of sialic acid in CLL lymphocytes.
|Number of pages||1|
|Journal||IRCS Medical Science|
|State||Published - 1985|