Reduced inhibition of replicon initiation and chain elongation by neocarzinostatin in skin fibroblasts from patients with ataxia telangiectasia

Yosef Shiloh*, Yechiel Becker

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Cells from patients with the genetic disease ataxia telangiectasia are hypersensitive to the DNA-breaking agents X-rays, bleomycin and neocarzinostatin, and show reduced inhibition of DNA synthesis after treatment with these agents, as compared to normal cells. The rate of replicon initiation and chain elongation was measured shortly after brief exposure of two normal and two ataxia telangiectasia fibroblast strains to low doses (0.10-0.30 μg/ml) of neocarzinostatin, by means of alkaline sucrose gradient analysis. Neocarzinostatin was found to inhibit both initiation and elongation, and both components of DNA synthesis were more resistant to this inhibition in the A-T strains.

Original languageEnglish
Pages (from-to)485-488
Number of pages4
JournalBBA - Molecular Cell Research
Volume721
Issue number4
DOIs
StatePublished - 30 Dec 1982
Externally publishedYes

Funding

FundersFunder number
Leonard Wolf son Foundation of the Hebrew University
Leukemia Research Foundation

    Keywords

    • (Fibroblast)
    • Ataxia telangiectasia
    • Chain elongation
    • Neocarzinostatin
    • Replicon initiation

    Fingerprint

    Dive into the research topics of 'Reduced inhibition of replicon initiation and chain elongation by neocarzinostatin in skin fibroblasts from patients with ataxia telangiectasia'. Together they form a unique fingerprint.

    Cite this