TY - JOUR
T1 - Reduced inhibition of replicon initiation and chain elongation by neocarzinostatin in skin fibroblasts from patients with ataxia telangiectasia
AU - Shiloh, Yosef
AU - Becker, Yechiel
N1 - Funding Information:
The authors wish to acknowledge the help of Professor G. Kohn, Department of Human Genetics, Hebrew University-Hadassah Medical Center, in whose laboratory the fibroblast strains were established. This study was supported by grants from The Leonard Wolf son Foundation of the Hebrew University and from the Leukemia Research Foundation, Chicago.
PY - 1982/12/30
Y1 - 1982/12/30
N2 - Cells from patients with the genetic disease ataxia telangiectasia are hypersensitive to the DNA-breaking agents X-rays, bleomycin and neocarzinostatin, and show reduced inhibition of DNA synthesis after treatment with these agents, as compared to normal cells. The rate of replicon initiation and chain elongation was measured shortly after brief exposure of two normal and two ataxia telangiectasia fibroblast strains to low doses (0.10-0.30 μg/ml) of neocarzinostatin, by means of alkaline sucrose gradient analysis. Neocarzinostatin was found to inhibit both initiation and elongation, and both components of DNA synthesis were more resistant to this inhibition in the A-T strains.
AB - Cells from patients with the genetic disease ataxia telangiectasia are hypersensitive to the DNA-breaking agents X-rays, bleomycin and neocarzinostatin, and show reduced inhibition of DNA synthesis after treatment with these agents, as compared to normal cells. The rate of replicon initiation and chain elongation was measured shortly after brief exposure of two normal and two ataxia telangiectasia fibroblast strains to low doses (0.10-0.30 μg/ml) of neocarzinostatin, by means of alkaline sucrose gradient analysis. Neocarzinostatin was found to inhibit both initiation and elongation, and both components of DNA synthesis were more resistant to this inhibition in the A-T strains.
KW - (Fibroblast)
KW - Ataxia telangiectasia
KW - Chain elongation
KW - Neocarzinostatin
KW - Replicon initiation
UR - http://www.scopus.com/inward/record.url?scp=0020364904&partnerID=8YFLogxK
U2 - 10.1016/0167-4889(82)90105-7
DO - 10.1016/0167-4889(82)90105-7
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0020364904
SN - 0167-4889
VL - 721
SP - 485
EP - 488
JO - BBA - Molecular Cell Research
JF - BBA - Molecular Cell Research
IS - 4
ER -