Recurrent functional misinterpretation of RNA-seq data caused by sample-specific gene length bias

Keyphrases

• Specific Genes 100%
• Misinterpretation 100%
• RNA-sequencing Data 100%
• Gene Length Bias 100%
• Sample-specific 100%
• Gene Set Enrichment Analysis 50%
• Length-biased 50%
• Normalization Method 33%
• RNA-seq Analysis 33%
• Gene Set 33%
• Gene Length 33%
• Extracellular Matrix Genes 33%
• Minimal Impact 16%
• Substantial Reduction 16%
• RNA Sequencing (RNA-seq) 16%
• False Positive Rate 16%
• Gene-gene 16%
• Systematic Effects 16%
• Statistical Power 16%
• Transcriptomic Data 16%
• Long Genes 16%
• Length Effect 16%
• M-value 16%
• Conditional Quantiles 16%
• Ribosomal Protein Genes 16%
• Technical Bias 16%
• Upper Quartile 16%
• False Calls 16%
• False Results 16%
• Quantile Normalization 16%
• Data Normalization 16%
• Trimmed Mean 16%
• Analysis pipeline 16%
• Transcript Length 16%
• Gene-gene Correlation 16%

Biochemistry, Genetics and Molecular Biology

• RNA Sequence 100%
• Genome-Wide Analysis of Gene Expression 50%
• Transcriptomics 25%
• Gene Set Enrichment 25%
• Ribosomal Protein 25%

Neuroscience

• RNA Sequence 100%
• Extracellular Matrix 50%
• Transcriptomics 25%
• Ribosome Protein 25%