TY - JOUR
T1 - Recovery from experimental allergic encephalomyelitis is TGF-β dependent and associated with increases in CD4+LAP+ and CD4+CD25+ T cells
AU - Zhang, Xingmin
AU - Reddy, Jayagopala
AU - Ochi, Hirofumi
AU - Frenkel, Dan
AU - Kuchroo, Vijay K.
AU - Weiner, Howard L.
PY - 2006/4
Y1 - 2006/4
N2 - SJL mice are highly susceptible to proteolipid protein (PLP) 139-151-induced experimental allergic encephalomyelitis (EAE). The disease is characterized by a relapsing-remitting type of paralysis. However, the mechanism by which animals recover from EAE is poorly understood. Here, we investigated the role of regulatory T cells in the recovery from disease. We found that Forkhead box P3-expressing CD4+CD25+ T cells were increased in the blood, draining lymph node and spleen of EAE-recovered SJL mice. These cells were anergic and inhibited proliferation of CD4+ CD25- T cells to PLP 139-151 or anti-CD3 antibody stimulation. Depletion of CD4-CD25+ T cells during the recovery phase exacerbated disease, resulted in the expansion of IAs/PLP 139-151-tetramer-positive cells and enhanced IFN-γ production. In addition, transforming growth factor-β (TGF-β) was shown to be involved in the recovery from EAE as the percentage of CD4+ cells expressing TGF-β latency-associated peptide (LAP) on the cell surface increased significantly in blood and spleen of EAE-recovered mice as compared with the naive mice and in vivo neutralization of TGF-β abolished recovery from disease. Taken together, our results demonstrate that both CD4+CD25+ and CD4+LAP+ regulatory T cells mediate recovery from PLP 139-151-induced EAE in SJL mice in which TGF-β plays an important role.
AB - SJL mice are highly susceptible to proteolipid protein (PLP) 139-151-induced experimental allergic encephalomyelitis (EAE). The disease is characterized by a relapsing-remitting type of paralysis. However, the mechanism by which animals recover from EAE is poorly understood. Here, we investigated the role of regulatory T cells in the recovery from disease. We found that Forkhead box P3-expressing CD4+CD25+ T cells were increased in the blood, draining lymph node and spleen of EAE-recovered SJL mice. These cells were anergic and inhibited proliferation of CD4+ CD25- T cells to PLP 139-151 or anti-CD3 antibody stimulation. Depletion of CD4-CD25+ T cells during the recovery phase exacerbated disease, resulted in the expansion of IAs/PLP 139-151-tetramer-positive cells and enhanced IFN-γ production. In addition, transforming growth factor-β (TGF-β) was shown to be involved in the recovery from EAE as the percentage of CD4+ cells expressing TGF-β latency-associated peptide (LAP) on the cell surface increased significantly in blood and spleen of EAE-recovered mice as compared with the naive mice and in vivo neutralization of TGF-β abolished recovery from disease. Taken together, our results demonstrate that both CD4+CD25+ and CD4+LAP+ regulatory T cells mediate recovery from PLP 139-151-induced EAE in SJL mice in which TGF-β plays an important role.
KW - EAE;LAP;Regulatory T cells;TGF-β
UR - http://www.scopus.com/inward/record.url?scp=33645225611&partnerID=8YFLogxK
U2 - 10.1093/intimm/dxh390
DO - 10.1093/intimm/dxh390
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AN - SCOPUS:33645225611
SN - 0953-8178
VL - 18
SP - 495
EP - 503
JO - International Immunology
JF - International Immunology
IS - 4
ER -