Recording Ca++ Transients in Neurons by TCSPC FLIM

Wolfgang Becker; Samuel Frere; Inna Slutsky

doi:10.1007/978-981-10-9020-2_5

Recording Ca⁺⁺ Transients in Neurons by TCSPC FLIM

Wolfgang Becker^*, Samuel Frere, Inna Slutsky

^*Corresponding author for this work

Physiology Pharmacology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

Multi-dimensional time-correlated single photon counting (TCSPC) techniques are able to record Ca⁺⁺ transients in live neurons via the fluorescence lifetime changes of a Ca⁺⁺-sensitive dye. The technique is based on periodical stimulation of the sample, raster scanning, or line scanning with a high-frequency pulsed laser, and multi-dimensional TCSPC. The recording process builds up a photon distribution over the spatial coordinates of the scan, the times of the photons in the laser pulse period, and the times of the photons in the stimulation period. The transient-time resolution is about 40 ms for raster scanning and about 1 ms for line scanning. We demonstrate the technique for electrical stimulation of cultured neurons incubated with Oregon Green Bapta.

Original language	English
Title of host publication	Progress in Optical Science and Photonics
Publisher	Springer
Pages	103-110
Number of pages	8
DOIs	https://doi.org/10.1007/978-981-10-9020-2_5
State	Published - 2019

Publication series

Name	Progress in Optical Science and Photonics
Volume	5
ISSN (Print)	2363-5096
ISSN (Electronic)	2363-510X

Keywords

Fluorescence Lifetime Imaging (FLIM)
Lifetime Changes
Oregon Green Bapta
Pulse-periodic Laser
Time-correlated Single Photon Counting (TCSPC)

Access to Document

10.1007/978-981-10-9020-2_5

Cite this

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abstract = "Multi-dimensional time-correlated single photon counting (TCSPC) techniques are able to record Ca++ transients in live neurons via the fluorescence lifetime changes of a Ca++-sensitive dye. The technique is based on periodical stimulation of the sample, raster scanning, or line scanning with a high-frequency pulsed laser, and multi-dimensional TCSPC. The recording process builds up a photon distribution over the spatial coordinates of the scan, the times of the photons in the laser pulse period, and the times of the photons in the stimulation period. The transient-time resolution is about 40 ms for raster scanning and about 1 ms for line scanning. We demonstrate the technique for electrical stimulation of cultured neurons incubated with Oregon Green Bapta.",

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