Reconstruction of genetic circuits

David Sprinzak; Michael B. Elowitz

doi:10.1038/nature04335

Reconstruction of genetic circuits

David Sprinzak, Michael B. Elowitz^*

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

294 Scopus citations

Abstract

The complex genetic circuits found in cells are ordinarily studied by analysis of genetic and biochemical perturbations. The inherent modularity of biological components like genes and proteins enables a complementary approach: one can construct and analyse synthetic genetic circuits based on their natural counterparts. Such synthetic circuits can be used as simple in vivo models to explore the relation between the structure and function of a genetic circuit. Here we describe recent progress in this area of synthetic biology, highlighting newly developed genetic components and biological lessons learned from this approach.

Original language	English
Pages (from-to)	443-448
Number of pages	6
Journal	Nature
Volume	438
Issue number	7067
DOIs	https://doi.org/10.1038/nature04335
State	Published - 24 Nov 2005
Externally published	Yes

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title = "Reconstruction of genetic circuits",

abstract = "The complex genetic circuits found in cells are ordinarily studied by analysis of genetic and biochemical perturbations. The inherent modularity of biological components like genes and proteins enables a complementary approach: one can construct and analyse synthetic genetic circuits based on their natural counterparts. Such synthetic circuits can be used as simple in vivo models to explore the relation between the structure and function of a genetic circuit. Here we describe recent progress in this area of synthetic biology, highlighting newly developed genetic components and biological lessons learned from this approach.",

author = "David Sprinzak and Elowitz, {Michael B.}",

note = "Funding Information: Acknowledgements Synthetic biology work in the laboratory is supported by a Burroughs-Wellcome CASI award, The HFSP program and the Searle Scholars program. D.S. would like to acknowledge the support of the Yad Hanadiv Foundation and the CBCD at Caltech. We would like to thank J. W. Chin, C. Guet, S. Leibler, J. Liao, W. Lim, C. Smolke and R. Weiss for contributing figures. We are grateful to R. Kishony, E. Sprinzak, C. Dalal, G. Suel and members of the laboratory for discussions and comments.",

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Reconstruction of genetic circuits

Abstract

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