TY - JOUR
T1 - Reconstitution of Photosynthetic Energy Conservation
T2 - II. Photophosphorylation in Liposomes Containing Photosystem‐I Reaction Center and Chloroplast Coupling‐Factor Complex
AU - HAUSKA, Gunter
AU - SAMORAY, Dietrich
AU - ORLICH, Gabriele
AU - NELSON, Nathan
PY - 1980/10
Y1 - 1980/10
N2 - Photophosphorylation has been reconstituted in a liposomal system containing reaction centers of photosystem I and coupling‐factor complex, both highly purified from spinach chloroplasts. This energy‐converting model system was put together by diluting the preparation of the coupling‐factor complex with an aqueous suspension of proteolipid vesicles, preformed from photosystem‐I reaction centers and soybean phospholipids by sonication. In the presence of reduced N‐methylphenazonium methosulfate the system catalyzed photophosphorylation with rates up to 50 μmol ATP formed × mg chlorophyll−1× h−1, which was sensitive to uncouplers and to N, N′‐dicyclohexylcarbodiimide. The properties of the system in comparison to chloroplasts is discussed.
AB - Photophosphorylation has been reconstituted in a liposomal system containing reaction centers of photosystem I and coupling‐factor complex, both highly purified from spinach chloroplasts. This energy‐converting model system was put together by diluting the preparation of the coupling‐factor complex with an aqueous suspension of proteolipid vesicles, preformed from photosystem‐I reaction centers and soybean phospholipids by sonication. In the presence of reduced N‐methylphenazonium methosulfate the system catalyzed photophosphorylation with rates up to 50 μmol ATP formed × mg chlorophyll−1× h−1, which was sensitive to uncouplers and to N, N′‐dicyclohexylcarbodiimide. The properties of the system in comparison to chloroplasts is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0019072386&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1980.tb04969.x
DO - 10.1111/j.1432-1033.1980.tb04969.x
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AN - SCOPUS:0019072386
SN - 0014-2956
VL - 111
SP - 535
EP - 543
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 2
ER -