Receptors for immune complexes on cells within a non lymphoid murine tumor

G. R. Braslawsky, M. Yaackubowicz, A. Frensdorff, I. P. Witz

Research output: Contribution to journalArticlepeer-review

Abstract

Evidence is presented that antibodies unrelated to the tumor cell can comprise part of the in vivo Ig surface coat of cells derived from the non lymphoid murine tumor, TA3/St. This was shown by incubating TA3 cells originating from other OA or BSA preimmunized mice with radioiodinated 125I OA and 131I BSA. Radioiodine labeled 125I OA specifically fixed to cells derived from TA3/St tumors originating from OA preimmunized mice. On the other hand 131I BSA was specifically fixed by cell populations from BSA preimmunized mice. Incubation of these cells in vitro at 37°C abolished the specific binding and antibody could subsequently be detected in the tissue culture medium. Radioiodine labeled purified soluble antibody antigen complexes could also be bound to cells derived from freshly harvested TA3/St tumors but not to their in vitro propagated counterparts. Removal of phagocytic or adherent cells from these cell populations decreased the binding of the complexed antibody on freshly harvested TA3/St populations, but did not eliminate it. Inhibition of complexed antibody binding was obtained when TA3/St cells (an H 2(a) tumor) were pre incubated with anti H 2(a) antiserum. Propagation of the tumor in an F1 hybrid (A x C57BL) in which host cells could be distinguished from tumor cells by using an anti H 2(b) antiserum showed that binding of the immune complex was mostly limited to host cells infiltrating into the tumor population.

Original languageEnglish
Pages (from-to)1571-1578
Number of pages8
JournalJournal of Immunology
Volume116
Issue number6
StatePublished - 1976

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