Receptor for activated C kinase (RACK) and protein kinase C (PKC) in egg activation

Yael Haberman, Lina Tsaadon Alon, Efrat Eliyahu, Ruth Shalgi

Research output: Contribution to journalArticlepeer-review

Abstract

In somatic cells, translocation of PKCs is facilitated by receptor for activated C kinase (RACK); however its involvement in egg activation is still elusive. We have followed the translocation pattern of conventional and novel PKCs (cPKCs and nPKCs, respectively) upon egg activation. Confocal microscopy indicated the expression and localization of RACK1, a specific receptor protein for cPKCs. Activation of MII eggs, led to translocation to the egg cortex of PKCα, βII and δ and the co-translocation of RACK1, with both PKCα and PKCβII. The association of PKC and actin, both known to be involved in cortical granules exocytosis (CGE) with RACK1, was demonstrated by co-immunoprecipitation. Egg activation resulted in an increased RACK1 level along with a decreased level of PKCβII. Based on these results, we suggest that upon egg activation, RACK1 shuttles activated cPKCs to the egg cortex, thus facilitating CGE.

Original languageEnglish
Pages (from-to)80-89
Number of pages10
JournalTheriogenology
Volume75
Issue number1
DOIs
StatePublished - 1 Jan 2011

Keywords

  • Fertilization
  • Parthenogenetic activation
  • Signal transduction

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