Real-time bioprocess monitoring using a mid-infrared fibre-optic sensor

Farah Alimagham*, James Winterburn, Ben Dolman, Patrícia Maia Domingues, Francesca Everest, Max Platkov, Svetlana Basov, Gregory Izakson, Abraham Katzir, Stephen R. Elliott, Tanya Hutter*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


This work proposes a simple, easy-to-fabricate and cost-efficient sensor based on mid-infrared (MIR) spectroscopy for in-situ real-time bioprocess monitoring. A process involving the production of sophorolipid biosurfactants by way of fermentation was studied with the aim of monitoring varying substrate and product concentrations over time and also distinguishing between the main two structurally different types of biosurfactant produced: lactonic and acidic sophorolipids. First, measurements were carried out using ATR-FTIR spectroscopy. This was followed by comparative measurements using a fabricated U-bent fibre-optic sensor designed for in-situ monitoring. It was demonstrated for the first time that varying concentrations of sophorolipids in a fermentation broth could successfully be monitored over time using an in-situ MIR fibre-optic sensor. Moreover, acidic and lactonic sophorolipids could simultaneously be identified at varying concentrations. The utilisation of such a sensor in bioprocesses can provide a wealth of real-time data, allowing for improved control of feeding rates and better process characterisation, providing significant opportunities for bioprocess control, resulting in increased production and decreased overall costs.

Original languageEnglish
Article number107889
JournalBiochemical Engineering Journal
StatePublished - Mar 2021


FundersFunder number
Engineering and Physical Sciences Research CouncilEP/L015889/1


    • Bioprocess monitoring
    • Biosurfactant
    • Fibre-optics
    • Mid-infrared spectroscopy
    • Sophorolipid


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