Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature

Ariel Munitz*, L. Edry-Botzer, M. Itan, R. Tur-Kaspa, D. Dicker, D. Marcoviciu, M. G. Goren, M. Mor, S. Lev, T. Gottesman, K. Muhsen, D. Cohen, M. Stein, U. Qimron, N. T. Freund, Y. Wine, Motti Gerlic*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Despite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies toward SARS-CoV-2 antigens, revealed a rapid onset of IgG/IgA antibodies, specifically in moderate/severe patients. IgM antibodies against the viral receptor binding domain, but not against nucleocapsid protein, were detected at early stages of the disease. Furthermore, we observed a marked reduction in IgM/IgA antibodies over-time. Adapting our assay for ACE2 binding-competition, demonstrated that the presence of potentially neutralizing antibodies is corelated with IgG/IgA. Finally, analysis of the cytokine profile in COVID-19 patients revealed unique correlation of an IL-12p70/IL33 and IgG seroconversion, which correlated with disease severity. In summary, our comprehensive analysis has major implications on the understanding and monitoring of SARS-CoV-2 infections.

Original languageEnglish
Article number3461
JournalScientific Reports
Volume11
Issue number1
DOIs
StatePublished - Dec 2021

Funding

FundersFunder number
Biological Industries
Cancer Biology Research Center
CoG-ERC1422/18
Israeli Innovation Authority65029
Milner Foundation1282/17, 2017359
Recanati Foundation
US-BSF2011244
Varda and Boaz Dotan Research Center in Hemato-Oncology
Alpha-1 Foundation2017176, 615533, 818/18
Alpha-1 Foundation
Israel Cancer Research Fund
Horizon 2020 Framework Programme
H2020 European Research Council818878
H2020 European Research Council
United States-Israel Binational Science Foundation
Israel Science Foundation886/15
Israel Science Foundation
Tel Aviv University
Varda and Boaz Dotan Research Center for Hemato-Oncology Research, Tel Aviv University

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