Rapid quantification of cellulose nanocrystals by Calcofluor White fluorescence staining

Roi Peretz, Hadas Mamane, Elizaveta Sterenzon, Yoram Gerchman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Abstract: Cellulose nanocrystals (CNCs) have gained increased interest worldwide for their unique properties. CNC production by acid hydrolysis of cellulose-rich biomass is well established, but its' quantification is still complicated. In this study, a rapid method for the determination of CNC concentration using Calcofluor White (CW) fluorescence dye is demonstrated for both purified and homemade CNCs recovered from Whatman filter paper. The method is robust, selective for crystalline cellulose, suitable for routine measurement in CNC production and that the pH must be basic for staining using CW. Graphical abstract: [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)971-977
Number of pages7
Issue number2
StatePublished - 30 Jan 2019


  • Calcofluor White
  • Cellulose nanocrystals (CNC)
  • Fluorescence
  • Whatman filter paper
  • quantification
  • quantification


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