Rapid phenotypic antimicrobial susceptibility testing using nanoliter arrays

Jonathan Avesar, Dekel Rosenfeld, Marianna Truman-Rosentsvit, Tom Ben-Arye, Yuval Geffen, Moran Bercovici, Shulamit Levenberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

118 Scopus citations

Abstract

Antibiotic resistance is a major global health concern that requires action across all sectors of society. In particular, to allow conservative and effective use of antibiotics clinical settings require better diagnostic tools that provide rapid determination of antimicrobial susceptibility. We present a method for rapid and scalable antimicrobial susceptibility testing using stationary nanoliter droplet arrays that is capable of delivering results in approximately half the time of conventional methods, allowing its results to be used the same working day. In addition, we present an algorithm for automated data analysis and a multiplexing system promoting practicality and translatability for clinical settings. We test the efficacy of our approach on numerous clinical isolates and demonstrate a 2-d reduction in diagnostic time when testing bacteria isolated directly from urine samples.

Original languageEnglish
Pages (from-to)E5787-E5795
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number29
DOIs
StatePublished - 18 Jul 2017
Externally publishedYes

Funding

FundersFunder number
I-core Israel Centers of Research Excellence
Israel Innovation Authority
Russell Berrie Nanotechnology Institute

    Keywords

    • Antibiotic resistance
    • Antibiotic susceptibility testing
    • Microfluidics
    • Nanoliter wells
    • Resazurin

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