Rapid identification of Streptococcus pyogenes by flow cytometry

E. Sahar*, R. Lamed, I. Ofek

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Flow cytometry combined with immunofluorescence of Streptococcus pyogenes was used to assay bacteria suspended in buffer solution and in saliva derived from throat swabs of healthy volunteers. The method allowed the enumeration of as few as 5 × 103 and 5 × 104 CFU per milliliter of buffer and saliva respectively. Controls including Streptococcus salivarius instead of Streptococcus pyogenes or buffer instead of specific antibodies confirmed the specificity of the detection of Streptococcus pyogenes in the samples. The results suggest that flow cytometry may serve as a basis for an automated reliable method for the diagnosis of streptococcal infections.

Original languageEnglish
Pages (from-to)192-195
Number of pages4
JournalEuropean Journal of Clinical Microbiology
Volume2
Issue number3
DOIs
StatePublished - Jun 1983

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