Rapid homogenous detection of the Ibaraki virus NS3 cDNA at picomolar concentrations by magnetic modulation

Amos Danielli*, Noga Porat, Ady Arie, Marcelo Ehrlich

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Magnetic modulation biosensing (MMB) system is experimentally demonstrated for rapid and homogeneous detection of the Ibaraki virus NS3 cDNA. A novel fluorescent resonance energy transfer (FRET)-based probe discriminates the target DNA from the control. When detection is made, the FRET-based probe is cleaved using Taq-polymerase activity and fluorescent light is produced. The biotinylated probes are attached to streptavidin-coupled superparamagnetic beads and are maneuvered into oscillatory motion by applying an alternating magnetic field gradient through two electromagnetic poles. The beads are condensed into the detection area and their movement in and out the orthogonal laser beam produces a periodic fluorescent signal that is demodulated using synchronous detection. 1.9 pM of the Ibaraki virus NS3 cDNA was detected in homogeneous solution within 18 min without separation or washing steps.

Original languageEnglish
Pages (from-to)858-863
Number of pages6
JournalBiosensors and Bioelectronics
Volume25
Issue number4
DOIs
StatePublished - 15 Dec 2009

Funding

FundersFunder number
Ishaia Horowitz Fund

    Keywords

    • DNA detection
    • Fluorescence and luminescence
    • Ibaraki virus
    • Magnetic beads
    • Magnetic modulation
    • Magnetic nanoparticles

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