Rapid diagnosis of congenital adrenal hyperplasia by high performance liquid chromatography

Yosef Weisman*, Ada Bar, Alien Root, Zvi Spirer, Abraham Golander

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This manuscript describes methods for the quantitation of serum concentrations of 17-hydroxyprogesterone and 11-deoxycortisol (Compound S) employing reversephase high-performance liquid chromatography (HPLC) and ultraviolet (UV) absorbance which are applicable to the diagnosis of congenital adrenal hyperplasia due to deficiencies of 21- and 11-hydroxylase activities, respectively. These methods are simple, specific, precise and rapid. Data obtained by the HPLC-UV methods are highly correlated (p < 0.001) with radioimmunoassay measurements.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalClinica Chimica Acta
Volume138
Issue number1
DOIs
StatePublished - 27 Mar 1984
Externally publishedYes

Keywords

  • Adrenal hyperplasia
  • HPLC
  • Serum corticosteroid

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