Rapid detection of influenza a pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR

Musa Hindiyeh; Daniela Ram; Michal Mandelboim; Tal Meningher; Shira Hirsh; Jana Robinov; Virginia Levy; Sara Orzitzer; Roberto Azar; Zehava Grossman; Ella Mendelson

doi:10.1128/JCM.02540-09

Rapid detection of influenza a pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR

Musa Hindiyeh^*
, Daniela Ram
, Michal Mandelboim
, Tal Meningher
, Shira Hirsh
, Jana Robinov
, Virginia Levy
, Sara Orzitzer
, Roberto Azar
, Zehava Grossman
, Ella Mendelson

^*Corresponding author for this work

School of Public Health

Sheba Medical Center at Tel Hashomer
Bar-Ilan University
Tel Aviv University

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.

Original language	English
Pages (from-to)	1884-1887
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	48
Issue number	5
DOIs	https://doi.org/10.1128/JCM.02540-09
State	Published - May 2010

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1128/JCM.02540-09

Cite this

Hindiyeh, M., Ram, D., Mandelboim, M., Meningher, T., Hirsh, S., Robinov, J., Levy, V., Orzitzer, S., Azar, R., Grossman, Z., & Mendelson, E. (2010). Rapid detection of influenza a pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR. Journal of Clinical Microbiology, 48(5), 1884-1887. https://doi.org/10.1128/JCM.02540-09

@article{dbf9191827ec458facec64357c1da663,

title = "Rapid detection of influenza a pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR",

abstract = "The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.",

author = "Musa Hindiyeh and Daniela Ram and Michal Mandelboim and Tal Meningher and Shira Hirsh and Jana Robinov and Virginia Levy and Sara Orzitzer and Roberto Azar and Zehava Grossman and Ella Mendelson",

year = "2010",

month = may,

doi = "10.1128/JCM.02540-09",

language = "אנגלית",

volume = "48",

pages = "1884--1887",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "5",

}

Hindiyeh, M, Ram, D, Mandelboim, M, Meningher, T, Hirsh, S, Robinov, J, Levy, V, Orzitzer, S, Azar, R, Grossman, Z & Mendelson, E 2010, 'Rapid detection of influenza a pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR', Journal of Clinical Microbiology, vol. 48, no. 5, pp. 1884-1887. https://doi.org/10.1128/JCM.02540-09

TY - JOUR

T1 - Rapid detection of influenza a pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR

AU - Hindiyeh, Musa

AU - Ram, Daniela

AU - Mandelboim, Michal

AU - Meningher, Tal

AU - Hirsh, Shira

AU - Robinov, Jana

AU - Levy, Virginia

AU - Orzitzer, Sara

AU - Azar, Roberto

AU - Grossman, Zehava

AU - Mendelson, Ella

PY - 2010/5

Y1 - 2010/5

N2 - The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.

AB - The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.

UR - https://www.scopus.com/pages/publications/77951842269

U2 - 10.1128/JCM.02540-09

DO - 10.1128/JCM.02540-09

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 20220153

AN - SCOPUS:77951842269

SN - 0095-1137

VL - 48

SP - 1884

EP - 1887

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Rapid detection of influenza a pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this