Raman spectroscopy complements optical coherent tomography in tissue classification and cancer detection

Ji Qi, Narendran Sudheendran, Chih Hao Liu, Greggy M. Santos, Eric D. Young, Alexander J. Lazar, Dina C. Lev, Raphael E. Pollock, Kirill V. Larin, Wei Chuan Shih*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Optical coherence tomography (OCT) provides significant advantages of high-resolution (approaching the histopathology level) real-time imaging of tissues without use of contrast agents. Based on these advantages, the microstructural features of tumors can be visualized and detected intra-operatively. However, it is still not clinically accepted for tumor margin delineation due to poor specificity and accuracy. In contrast, Raman spectroscopy (RS) can obtain tissue information at the molecular level, but does not provide real-time imaging capability. Therefore, combining OCT and RS could provide synergy. To this end, we present a tissue analysis and classification method using both the slope of OCT intensity signal versus depth and the principle components from the RS spectrum as the indicators for tissue characterization. Our pilot experiments were performed on mouse kidneys, livers, and small intestines. The prediction accuracy with five-fold cross validation of the method has been evaluated by support vector machine method. The results demonstrate that RS can effectively improve tissue classification compared to OCT alone. Next, we demonstrate that the boundary between myxoid liposarcoma and normal fat which is easily identifiable both Raman and OCT. In cases where structural images are indistinguishable, for example, in normal fat and well differentiated liposarcoma (WDLS) or gastrointestinal sarcoma tumor (GIST) and Myxoma, distinct molecular spectra have been obtained. The results suggest RS can effectively complement OCT to tumor boundary demarcation with high specificity.

Original languageEnglish
Title of host publicationOptical Biopsy XIII
Subtitle of host publicationToward Real-Time Spectroscopic Imaging and Diagnosis
EditorsRobert R. Alfano, Stavros G. Demos
ISBN (Electronic)9781628414080
StatePublished - 2015
Externally publishedYes
EventOptical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis - San Francisco, United States
Duration: 10 Feb 201511 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceOptical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Country/TerritoryUnited States
CitySan Francisco


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