TY - JOUR
T1 - Radiation sterilization of triple sugar iron agar
AU - Altmann, G.
AU - Eisenberg, E.
AU - Bogokowsky, Bianca
N1 - Funding Information:
Acknowled#ement--This study was supported by a grant from Isotope Radiation Enterprises Ltd, Tel Aviv, Israel.
PY - 1979/9
Y1 - 1979/9
N2 - Triple sugar iron agar (TSI), a medium used for the identification of enteric bacteria, was sterilized by gamma radiation using radiation doses of 750-2000 krad. The radio-sterilized medium, slightly modified by increasing its Phenol Red content, performed well when tested with different enterobacteria-ceae and other gram negative bacteria. Growth, change of indicator reaction in slant and butt and formation of gas and H2S were equal in irradiated and autoclaved TSI. Slants of irradiated TSI in stoppered plastic tubes kept their diagnostic properties during storage for at least 4 months. Gamma irradiation appears to be an attractive and economical method of sterilizing nutrient media in sealed tubes or other containers, avoiding the risk of contamination during processing.
AB - Triple sugar iron agar (TSI), a medium used for the identification of enteric bacteria, was sterilized by gamma radiation using radiation doses of 750-2000 krad. The radio-sterilized medium, slightly modified by increasing its Phenol Red content, performed well when tested with different enterobacteria-ceae and other gram negative bacteria. Growth, change of indicator reaction in slant and butt and formation of gas and H2S were equal in irradiated and autoclaved TSI. Slants of irradiated TSI in stoppered plastic tubes kept their diagnostic properties during storage for at least 4 months. Gamma irradiation appears to be an attractive and economical method of sterilizing nutrient media in sealed tubes or other containers, avoiding the risk of contamination during processing.
UR - http://www.scopus.com/inward/record.url?scp=0018648107&partnerID=8YFLogxK
U2 - 10.1016/0020-708X(79)90165-0
DO - 10.1016/0020-708X(79)90165-0
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AN - SCOPUS:0018648107
SN - 0020-708X
VL - 30
SP - 527
EP - 529
JO - Applied Radiation and Isotopes
JF - Applied Radiation and Isotopes
IS - 9
ER -