Radiation sterilization of triple sugar iron agar

G. Altmann*, E. Eisenberg, Bianca Bogokowsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Triple sugar iron agar (TSI), a medium used for the identification of enteric bacteria, was sterilized by gamma radiation using radiation doses of 750-2000 krad. The radio-sterilized medium, slightly modified by increasing its Phenol Red content, performed well when tested with different enterobacteria-ceae and other gram negative bacteria. Growth, change of indicator reaction in slant and butt and formation of gas and H2S were equal in irradiated and autoclaved TSI. Slants of irradiated TSI in stoppered plastic tubes kept their diagnostic properties during storage for at least 4 months. Gamma irradiation appears to be an attractive and economical method of sterilizing nutrient media in sealed tubes or other containers, avoiding the risk of contamination during processing.

Original languageEnglish
Pages (from-to)527-529
Number of pages3
JournalApplied Radiation and Isotopes
Volume30
Issue number9
DOIs
StatePublished - Sep 1979
Externally publishedYes

Funding

FundersFunder number
Isotope Radiation Enterprises Ltd

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